Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.
Chemosphere. 2013 Aug;92(10):1314-20. doi: 10.1016/j.chemosphere.2013.05.009. Epub 2013 May 31.
The occurrence of Accumulibacter Type I (a known phosphorus-accumulating organism, PAO) has received increased attention due to the potential operating benefits associated with their denitrifying activity in enhanced biological phosphorus removal (EBPR) wastewater treatment plants. In this study, after a shift from an enriched glycogen-accumulating organism (GAO) culture (competitors of PAO) to a PAO-enriched system, Accumulibacter Type I (PAO I) became dominant in an anaerobic-aerobic EBPR system fed with acetate and operated at 10°C with a net aerobic solids retention time (SRT) of 6 d. Since Accumulibacter Type II (PAO II) were not detected, the low temperature in combination with the net aerobic SRT applied appeared to have suppressed their growth as well. The stoichiometry of PAO I was in agreement with previous metabolic models, suggesting that it was the main PAO organisms present in previous studies operated under similar conditions. Moreover, under poly-P limiting conditions, PAO I were unable to switch to a GAO-like metabolism at low temperatures. These results contribute to increase the understanding of the physiology, microbial metabolism and microbial ecology of PAO I.
由于具有反硝化活性的 Accumulibacter Type I（一种已知的聚磷菌，PAO）在强化生物除磷（EBPR）污水处理厂中具有潜在的运行优势，因此其受到了越来越多的关注。在本研究中，从富含糖原的聚磷菌（GAO）培养物（PAO 的竞争菌）到富含 PAO 的系统转变后，Accumulibacter Type I（PAO I）在以乙酸盐为食料、在 10°C 下运行且净好氧固体停留时间（SRT）为 6d 的厌氧-好氧 EBPR 系统中占据主导地位。由于未检测到 Accumulibacter Type II（PAO II），低温与所施加的净好氧 SRT 的结合似乎也抑制了它们的生长。PAO I 的化学计量与之前的代谢模型一致，这表明在类似条件下运行的先前研究中，它是主要的 PAO 生物。此外，在多聚磷酸盐（poly-P）限制条件下，PAO I 无法在低温下切换到类似 GAO 的代谢。这些结果有助于提高对 PAO I 的生理学、微生物代谢和微生物生态学的理解。
