Institute of Sciences of Food Production, ISPA-CNR, Via Amendola 122/0, 70126, Bari, Italy.
Int J Food Microbiol. 2013 Jul 15;165(2):163-8. doi: 10.1016/j.ijfoodmicro.2013.04.027. Epub 2013 May 14.
Dried vine fruits may be heavily colonized by Aspergillus species. The molecular biodiversity of an Aspergillus population (234 strains) isolated from dried vine fruit samples of worldwide origin were analyzed by investigating four housekeeping gene loci (calmodulin, β-tubulin, elongation factor 1-α, RPB2). Aspergillus Sect. Nigri was dominant and the strains were identified as A. tubingensis (138), A. awamori (38), A. carbonarius (27), A. uvarum (16) and A. niger (11). Four Aspergillus flavus strains were also identified from Chilean raisins. Two clusters closely related to the A. tubingensis species with a significant bootstrap (60% and 99%) were identified as distinct populations. Among the four loci, RPB2 showed the highest genetic variability. This is the first complete study on the worldwide distribution of black Aspergilli occurring on dried vine fruits identified by a molecular approach.
干葡萄干可能被曲霉属（Aspergillus）严重定植。通过研究四个管家基因座（钙调蛋白、β-微管蛋白、延伸因子 1-α、RPB2），分析了来自世界各地干葡萄干样本的曲霉种群（234 株）的分子生物多样性。曲霉属 Nigri 节占优势，菌株被鉴定为 A. tubingensis（138 株）、A. awamori（38 株）、A. carbonarius（27 株）、A. uvarum（16 株）和 A. niger（11 株）。还从智利葡萄干中鉴定出了 4 株黄曲霉（Aspergillus flavus）菌株。两个与 A. tubingensis 种密切相关的聚类，支持率分别为 60%和 99%，被鉴定为不同的种群。在四个基因座中，RPB2 显示出最高的遗传变异性。这是首次通过分子方法对发生在干葡萄干上的黑曲霉属（Aspergillus）在全球的分布进行的完整研究。
