Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2013 Aug;104(2):225-31. doi: 10.1007/s10482-013-9941-1. Epub 2013 Jun 4.
A Gram-stain-negative, non-spore-forming, aerobic, non-flagellated, gliding and rod-shaped bacterial strain, designated HJ-26M(T), was isolated from a tidal flat sediment in the Korean peninsula. It grew optimally at 25-30 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that the strain fell within the clade comprising Tenacibaculum species, clustering coherently with the type strains of Tenacibaculum lutimaris and Tenacibaculum aestuarii. Strain HJ-26M(T) exhibited the highest 16S rRNA gene sequence similarity values of 98.4 and 98.2 % to T. lutimaris TF-26(T) and T. aestuarii SMK-4(T), respectively, and of 94.9-97.4 % to the type strains of the other Tenacibaculum species. Strain HJ-26M(T) contained MK-6 as the predominant menaquinone and iso-C15:0 and iso-C17:0 3-OH as the major fatty acids. The DNA G+C content of strain HJ-26M(T) was 34.5 mol% and its mean DNA-DNA relatedness values with the type strains of T. lutimaris and T. aestuarii were 19 and 23 %, respectively. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain HJ-26M(T) is separate from other Tenacibaculum species. On the basis of the data presented, strain HJ-26M(T) is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum caenipelagi sp. nov. is proposed. The type strain is HJ-26M(T) (= KCTC 32323(T) = CECT 8283(T)).
一株革兰氏阴性、非孢子形成、需氧、无鞭毛、滑行和杆状的细菌菌株，被命名为 HJ-26M(T)，从朝鲜半岛的潮滩沉积物中分离得到。它在 25-30°C、pH 值 7.0-8.0 和 2%（w/v）NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的邻接法系统发育树显示，该菌株属于 Tenacibaculum 属的一个分支，与 Tenacibaculum lutimaris 和 Tenacibaculum aestuarii 的模式菌株聚为一簇。菌株 HJ-26M(T)与 T. lutimaris TF-26(T)和 T. aestuarii SMK-4(T)的 16S rRNA 基因序列相似性最高，分别为 98.4%和 98.2%，与其他 Tenacibaculum 种的模式菌株的相似性为 94.9-97.4%。菌株 HJ-26M(T)含有 MK-6 作为主要的类异戊二烯醌，iso-C15:0 和 iso-C17:0 3-OH 作为主要的脂肪酸。菌株 HJ-26M(T)的 DNA G+C 含量为 34.5mol%，与 T. lutimaris 和 T. aestuarii 模式菌株的平均 DNA-DNA 同源性分别为 19%和 23%。表型特征的差异，以及系统发育和遗传上的独特性，表明菌株 HJ-26M(T)与其他 Tenacibaculum 种不同。基于所提供的数据，菌株 HJ-26M(T)被认为代表 Tenacibaculum 属的一个新种，命名为 Tenacibaculum caenipelagi sp. nov.。模式菌株为 HJ-26M(T)=KCTC 32323(T)=CECT 8283(T)。
