Department of Agronomy & James D. Watson Institute of Genome Sciences; Zhejiang University; Hangzhou, PR China.
Plant Signal Behav. 2013 Aug;8(8). doi: 10.4161/psb.25079. Epub 2013 Jun 26.
Plant miRNAs can trigger the production of phased, secondary siRNAs from either non-coding or protein-coding genes. In this study, at least 864 and 3,961 loci generating 21-nt and 24-nt phased siRNAs (phasiRNAs),respectively, were identified in three tissues from wild rice. Of these phasiRNA-producing loci, or PHAS genes, biogenesis of phasiRNAs in at least 160 of 21-nt and 254 of 24-nt loci could be triggered by interaction with miRNA(s). Developing seeds had more PHAS genes than leaves and roots. Genetic constrain on miRNA-triggered PHAS genes suggests that phasiRNAs might be one of the driving forces contributed to rice domestication.
植物 microRNA 可以从非编码或编码基因中引发有特定顺序的二级 siRNA 的产生。在这项研究中,在野生稻的三种组织中分别鉴定出了至少 864 和 3961 个产生 21 个核苷酸和 24 个核苷酸相匹配 siRNA(phasiRNA)的基因座。在这些产生 phasiRNA 的基因座或 PHAS 基因中,至少有 21 个核苷酸的 160 个和 24 个核苷酸的 254 个基因座的 phasiRNA 生物合成可以被 miRNA 的相互作用触发。发育中的种子比叶片和根具有更多的 PHAS 基因。miRNA 触发的 PHAS 基因的遗传限制表明,phasiRNA 可能是促成水稻驯化的驱动力之一。