Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.
Pathog Dis. 2013 Aug;68(3):78-81. doi: 10.1111/2049-632X.12047. Epub 2013 Jun 26.
The formation of intracellular bacterial communities (IBC) has been proposed as a new pathogenic model for urinary tract infections. Scarce reports describe this phenomenon in humans. We describe the presence of IBC in uroepithelial cells of a child with recurrent urinary infections. Urine specimen was collected from a child with Escherichia coli UTI and analyzed by light and confocal laser scanning microscopy (CLSM). The capability of this strain to produce intracellular infection in bladder tissue was confirmed in mice models. Escherichia coli phylogenetic group, presence of virulence factors genes, and its multiple locus sequence type were determined. CLSM showed large collections of morphologically coccoid and rod bacteria in eukaryotic cells cytoplasm, even seemingly protruding from the cells. Escherichia coli EC7U, ST3626, harbored type 1, P, and S/F1C fimbriae and K1 capsule genes. In this report, we confirm the presence of IBC in children with UTI, as it has been described before in women.
细胞内细菌群落（IBC）的形成已被提出作为尿路感染的一种新的致病模型。关于这种现象在人类中的描述很少。我们描述了一名复发性尿路感染患儿的尿路上皮细胞中存在 IBC。从患有大肠埃希菌尿路感染的儿童中采集尿液标本，并通过光学显微镜和共聚焦激光扫描显微镜（CLSM）进行分析。该菌株在小鼠模型中产生膀胱组织内感染的能力得到了证实。确定了大肠埃希菌的进化群、毒力因子基因的存在及其多位点序列型。CLSM 显示大量形态学上呈球菌和杆状细菌的集合存在于真核细胞的细胞质中，甚至似乎从细胞中突出。EC7U、ST3626 大肠埃希菌携带 1 型、P 型和 S/F1C 菌毛以及 K1 荚膜基因。在本报告中，我们证实了 UTI 患儿存在 IBC，正如之前在女性中所描述的那样。
