Chatterjea Anindita, van der Stok Johan, Danoux Charlène B, Yuan Huipin, Habibovic Pamela, van Blitterswijk Clemens A, Weinans Harrie, de Boer Jan
Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, the Netherlands.
J Biomed Mater Res A. 2014 May;102(5):1399-407. doi: 10.1002/jbm.a.34815. Epub 2013 Jun 12.
In the present study, two open porous calcium phosphate ceramics, β-tricalcium phosphate (β-TCP), and hydroxyapatite (HA) were compared in a critical-sized femoral defect in rats. Previous comparisons of these two ceramics showed significantly greater osteoinductive potential of β-TCP upon intramuscular implantation and a better performance in a spinal fusion model in dogs. Results of the current study also showed significantly more bone formation in defects grafted with β-TCP compared to HA; however, both the ceramics were not capable of increasing bone formation to such extend that it bridges the defect. Furthermore, a more pronounced degradation of β-TCP was observed as compared to HA. Progression of inflammation and initiation of new bone formation were assessed for both materials at multiple time points by histological and fluorochrome-based analyses. Until 12 days postimplantation, a strong inflammatory response in absence of new bone formation was observed in both ceramics, without obvious differences between the two materials. Four weeks postimplantation, signs of new bone formation were found in both β-TCP and HA. At 6 weeks, inflammation had subsided in both ceramics while bone deposition continued. In conclusion, the two ceramics differed in the amount of bone formed after 8 weeks of implantation, whereas no differences were found in the duration of the inflammatory phase after implantation or initiation of new bone formation.
在本研究中，对两种开孔多孔磷酸钙陶瓷——β-磷酸三钙（β-TCP）和羟基磷灰石（HA）——在大鼠临界尺寸股骨缺损模型中进行了比较。此前对这两种陶瓷的比较显示，β-TCP在肌肉内植入时具有显著更强的骨诱导潜力，并且在犬类脊柱融合模型中表现更佳。本研究结果还表明，与HA相比，β-TCP植入的缺损部位骨形成明显更多；然而，两种陶瓷均无法使骨形成增加到足以桥接缺损的程度。此外，与HA相比，观察到β-TCP的降解更为明显。通过组织学和基于荧光染料的分析，在多个时间点对两种材料的炎症进展和新骨形成起始情况进行了评估。植入后12天内，在两种陶瓷中均观察到强烈的炎症反应且无新骨形成，两种材料之间无明显差异。植入后四周，在β-TCP和HA中均发现了新骨形成的迹象。在6周时，两种陶瓷中的炎症均已消退，而骨沉积仍在继续。总之，两种陶瓷在植入8周后形成的骨量有所不同，而在植入后炎症期的持续时间或新骨形成起始方面未发现差异。
