Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville Campus, 381 Royal Parade, Parkville, VIC, 3052, Australia.
AAPS PharmSciTech. 2013 Sep;14(3):927-34. doi: 10.1208/s12249-013-9976-7. Epub 2013 Jun 4.
There is increasing attention in the literature towards understanding the behaviour of lipid-based drug formulations under digestion conditions using in vitro and in vivo methods. This necessitates a convenient method for quantitation of lipids and lipid digestion products. In this study, a simple and accessible method for the separation and quantitative determination of typical formulation and digested lipids using high performance liquid chromatography coupled to refractive index detection (HPLC-RI) is described. Long and medium chain lipids were separated and quantified in a biological matrix (gastrointestinal content) without derivatisation using HPLC-RI on C18 and C8 columns, respectively. The intra- and inter-assay accuracy was between 92% and 106%, and the assays were precise to within a coefficient of variation of less than 10% over the range of 0.1-2 mg/mL for both long and medium chain lipids. This method is also shown to be suitable for quantifying the lipolysis products collected from the gastrointestinal tract in the course of in vivo lipid digestion studies.
文献中越来越关注使用体内和体外方法来理解脂质药物制剂在消化条件下的行为。这需要一种方便的方法来定量脂质和脂质消化产物。本研究描述了一种简单易用的方法，可使用高效液相色谱法结合折射指数检测（HPLC-RI）分离和定量典型制剂和消化脂质。使用 HPLC-RI 在 C18 和 C8 柱上分别分离和定量长链和中链脂质，无需衍生化，可在生物基质（胃肠道内容物）中进行。长链和中链脂质的内和日间准确度在 92%至 106%之间，在 0.1-2mg/mL 范围内，变异系数小于 10%。该方法也适用于定量体内脂质消化研究过程中从胃肠道收集的脂肪分解产物。
