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一种多模式分析方法，用于在常规脂质组学中同时测定生物样品中的单乙酰二酰基甘油、中长链甘油三酯。

A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics.

机构信息

School of Science and the Environment/ Boreal Ecosystem Research Initiative, Grenfell Campus, Memorial University of Newfoundland, Corner Brook, NL, A2H 5G4, Canada.

出版信息

Lipids Health Dis. 2022 May 10;21(1):42. doi: 10.1186/s12944-022-01650-w.

DOI:10.1186/s12944-022-01650-w
PMID:35538477
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9092795/
Abstract

BACKGROUND

Monoacetyldiglycerides (MAcDG), are acetylated triglycerides (TG) and an emerging class of bioactive or functional lipid with promising nutritional, medical, and industrial applications. A major challenge exists when analyzing MAcDG from other subclasses of TG in biological matrices, limiting knowledge on their applications and metabolism.

METHODS

Herein a multimodal analytical method for resolution, identification, and quantitation of MAcDG in biological samples was demonstrated based on thin layer chromatography-flame ionization detection complimentary with C30-reversed phase liquid chromatography-high resolution accurate mass tandem mass spectrometry. This method was then applied to determine the MAcDG molecular species composition and quantity in E. solidaginis larvae. The statistical method for analysis of TG subclass composition and molecular species composition of E. solidaginis larvae was one-way analysis of variance (ANOVA).

RESULTS

The findings suggest that the proposed analytical method could simultaneously provide a fast, accurate, sensitive, high throughput analysis of MAcDG from other TG subclasses, including the fatty acids, isomers, and molecular species composition.

CONCLUSION

This method would allow for MAcDG to be included during routine lipidomics analysis of biological samples and will have broad interests and applications in the scientific community in areas such as nutrition, climate change, medicine and biofuel innovations.

摘要

背景

单乙酰化甘油二酯（MAcDG）是甘油三酯（TG）的乙酰化产物，是一类具有生物活性或功能的新型脂质，在营养、医学和工业领域具有广阔的应用前景。然而，在分析生物基质中 MAcDG 与其他 TG 亚类时，存在一个主要挑战，这限制了人们对其应用和代谢的了解。

方法

本研究建立了一种基于薄层色谱-火焰离子化检测（TLC-FID）与 C30 反相液相色谱-高分辨精确质量串联质谱（LC-HRMS/MS）联用的多模式分析方法，用于鉴定和定量分析生物样品中的 MAcDG。然后，该方法应用于测定美洲黑杨尺蠖幼虫中 MAcDG 的分子种类组成和含量。采用单因素方差分析（ANOVA）对美洲黑杨尺蠖幼虫 TG 亚类组成和分子种类组成进行统计分析。

结果

研究结果表明，所提出的分析方法能够快速、准确、灵敏、高通量地分析其他 TG 亚类中的 MAcDG，包括脂肪酸、异构体和分子种类组成。

结论

该方法将允许在生物样品的常规脂质组学分析中包括 MAcDG，并将在营养、气候变化、医学和生物燃料创新等领域的科学界中具有广泛的兴趣和应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/bd9b352efab9/12944_2022_1650_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/c9e98606e18a/12944_2022_1650_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/fa4510d3b023/12944_2022_1650_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/c493d8de09f7/12944_2022_1650_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/1e8b50a564e8/12944_2022_1650_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/ec0bf421d1b5/12944_2022_1650_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/deaf88450cfe/12944_2022_1650_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/bd9b352efab9/12944_2022_1650_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/c9e98606e18a/12944_2022_1650_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/fa4510d3b023/12944_2022_1650_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/c493d8de09f7/12944_2022_1650_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/1e8b50a564e8/12944_2022_1650_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/ec0bf421d1b5/12944_2022_1650_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/deaf88450cfe/12944_2022_1650_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d9/9092795/bd9b352efab9/12944_2022_1650_Fig7_HTML.jpg

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