Sun Qiang, Overholtzer Michael
Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
Methods Mol Biol. 2013;1004:59-66. doi: 10.1007/978-1-62703-383-1_5.
Entosis is a recently described nonapoptotic cell death mechanism that is initiated by the engulfment of live epithelial cells, leading to the formation of "cell-in-cell" structures. Entotic cell engulfment is induced by matrix detachment, and is driven by imbalances in actomyosin contraction between neighboring cells. Here we describe methods to quantify the formation of cell-in-cell structures by entosis, for cells cultured in suspension or in soft agar, by fluorescence imaging and time-lapse microscopy.
内吞作用是一种最近被描述的非凋亡性细胞死亡机制，它由活上皮细胞的吞噬作用引发，导致“细胞内细胞”结构的形成。内吞性细胞吞噬作用由基质脱离诱导，并由相邻细胞之间肌动球蛋白收缩的失衡驱动。在这里，我们描述了通过荧光成像和延时显微镜，对悬浮培养或软琼脂培养的细胞中因内吞作用形成的细胞内细胞结构进行量化的方法。
