Foo Lynette C
Department of Neurobiology, School of Medicine, Stanford University, Stanford, California 94305, USA.
Cold Spring Harb Protoc. 2013 Jun 1;2013(6):485-7. doi: 10.1101/pdb.top070912.
For years, studies of neural-glial interactions have relied on the use of astrocytes derived from the extended culture of immature precursor cells isolated from the neonatal rodent brain. Although the astrocytes cultured under these selective cell survival conditions have been important tools for understanding astrocyte behavior, they do not necessarily reflect the behavior and function of mature astrocytes. We have developed methods for acute, prospective isolation and culture of mature astrocytes from rodent brains in a serum-free, defined medium. These immunopanning-based methods facilitate the study of astrocyte biology and function.
多年来，神经胶质细胞相互作用的研究一直依赖于使用从新生啮齿动物大脑中分离出的未成熟前体细胞进行长期培养所获得的星形胶质细胞。尽管在这些选择性细胞存活条件下培养的星形胶质细胞一直是理解星形胶质细胞行为的重要工具，但它们不一定能反映成熟星形胶质细胞的行为和功能。我们已经开发出了在无血清、特定培养基中从啮齿动物大脑中急性、前瞻性地分离和培养成熟星形胶质细胞的方法。这些基于免疫淘选的方法有助于对星形胶质细胞生物学和功能的研究。
