细胞分裂分析的相关光镜和电镜技术

Correlative light and electron microscopy for the analysis of cell division.

机构信息

Structural Cell Biology Group, Experimental Center, Medical Faculty Carl Gustav Carus, University of Technology Dresden, Dresden 01307, Germany.

出版信息

J Microsc. 2013 Aug;251(2):109-12. doi: 10.1111/jmi.12056. Epub 2013 Jun 4.

DOI:10.1111/jmi.12056

PMID:23734865

Abstract

Correlative light and electron microscopy (CLEM) has recently gained increasing attention, because it enables the acquisition of dynamic as well as ultrastructural information about subcellular processes. It is the power of combining the two imaging modalities that gives additional information as compared to using the imaging techniques separately. Here, we briefly summarize two CLEM approaches for the analysis of cells in mitosis and cytokinesis.

摘要

相关光镜和电子显微镜（CLEM）最近受到越来越多的关注，因为它能够获得关于亚细胞过程的动态和超微结构信息。与单独使用成像技术相比，将两种成像模式结合使用会提供更多的信息。在这里，我们简要总结了两种用于分析有丝分裂和胞质分裂细胞的 CLEM 方法。