Department of Environmental, Agricultural & Occupational Health, University of Nebraska Medical Center College of Public Health, Omaha , NE 68198-4320, USA.
Prehosp Emerg Care. 2013 Jul-Sep;17(3):401-8. doi: 10.3109/10903127.2013.792889.
We evaluated gaseous chlorine dioxide (ClO2) decontamination of an ambulance using a variety of bacterial biological agents.
Spores of attenuated Bacillus anthracis and Bacillus atrophaeus as well as vegetative cells of Acinetobacter baumannii, Mycobacterium smegmatis, and Staphylococcus aureus were exposed to ClO2 gas inside an ambulance. Log reduction in viability was assessed following decontamination using organism plate counts.
Ambulance decontamination with ClO2 gas concentrations of 362 to 695 ppm maintained to exposures of 756 ppm-hours with 65% relative humidity (RH) achieved inactivation of all the bacterial agents tested. Decreasing exposure (ppm-hours) and RH (<65%) or restricting air flow reduced inactivation but still achieved greater than 6-log reductions in organism viability.
Up to 10-log reductions were achieved in an ambulance interior following exposure to ClO2, indicating that gas concentrations needed to mitigate biological agent contamination can be achieved and maintained safely in an ambulance. Future studies are ongoing to evaluate gaseous ClO2 in other environments contaminated with biological agents of health care concern.
我们使用各种细菌生物制剂评估了救护车中气态二氧化氯（ClO2）的消毒效果。
将衰减炭疽芽孢杆菌和萎缩芽孢杆菌的孢子以及鲍曼不动杆菌、耻垢分枝杆菌和金黄色葡萄球菌的营养细胞暴露于救护车内部的 ClO2 气体中。使用生物平板计数法评估消毒后的存活能力的对数减少情况。
在 65%相对湿度（RH）下，ClO2 气体浓度为 362 至 695 ppm 且暴露时间为 756 ppm-hours 的情况下，救护车消毒可使所有测试的细菌制剂失活。降低暴露（ppm-hours）和 RH（<65%）或限制空气流动会降低失活率，但仍可使生物制剂的存活能力减少 6 个对数以上。
在救护车内部暴露于 ClO2 后，可实现高达 10 个对数的减少，表明可以在救护车中安全地达到并维持消除生物制剂污染所需的气体浓度。未来的研究正在评估其他受医疗保健相关生物制剂污染的环境中的气态 ClO2。
