Ling Yang, Yin-Shi Guo, Hong-Wei Shan, Yan Zhou, Qiang Li, Ye Tian, Ying Xiong
Department of Geriatrics, Xinhua Hospital .
J Matern Fetal Neonatal Med. 2014 Jan;27(2):192-6. doi: 10.3109/14767058.2013.811228. Epub 2013 Jul 9.
Our objective was to assess the oxidative stress status and analyse the relationship between an oxidant/antioxidant imbalance and the mediator release properties of cord blood basophils from neonates born to mothers with atopic asthma.
Cord blood was collected from the neonates of 16 asthmatic mothers and 18 healthy mothers Basophils were purified and stimulated by Dermatophagoides farinae (Df), hyperosmotic mannitol or peptidoglycan (PGN). Immunoblotting detected nuclear factor κB (NFκB) as a measure of functional receptor response. The linear correlations between IL-4 levels in the supernatants and 3-nitrotyrosine (3-NT), glutathione peroxidase (GSH-Px) in the serum were evaluated.
Compared with the healthy group, the levels of 3-NT in maternal blood and cord blood were significantly higher in allergic asthma group, whereas the GSH-Px activity were lower. After stimulation, cord blood basophils from the neonates born to atopic mothers produced more IL-4 involving NF-κB pathways. There was a significant relationship between the IL-4 levels produced by basophils and 3-NT (or GSH-Px) in cord blood from allergic asthma group.
In asthma, mediator release properties of human basophils induced by environmental allergens and irritants are associated with oxidative stress, which may be one of the pathogenesis of allergic diseases.
我们的目的是评估氧化应激状态，并分析氧化/抗氧化失衡与患有特应性哮喘母亲所生新生儿脐带血嗜碱性粒细胞介质释放特性之间的关系。
从16名哮喘母亲和18名健康母亲的新生儿中采集脐带血。纯化嗜碱性粒细胞，并用粉尘螨（Df）、高渗甘露醇或肽聚糖（PGN）进行刺激。免疫印迹法检测核因子κB（NFκB），作为功能性受体反应的指标。评估上清液中白细胞介素-4（IL-4）水平与血清中3-硝基酪氨酸（3-NT）、谷胱甘肽过氧化物酶（GSH-Px）之间的线性相关性。
与健康组相比，过敏性哮喘组母亲血液和脐带血中3-NT水平显著升高，而GSH-Px活性较低。刺激后，患有特应性母亲所生新生儿的脐带血嗜碱性粒细胞通过NF-κB途径产生更多的IL-4。过敏性哮喘组脐带血中嗜碱性粒细胞产生的IL-4水平与3-NT（或GSH-Px）之间存在显著关系。
在哮喘中，环境过敏原和刺激物诱导的人嗜碱性粒细胞介质释放特性与氧化应激有关，这可能是过敏性疾病的发病机制之一。
