Yang Ling, Guo Yin-Shi, Jiang Jin-Qi, Guo Xue-Jun, Xu Yi-Ping, Tian Ye, Xiong Ying, Han Li
Geriatrics Department, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, China.
Hybridoma (Larchmt). 2012 Aug;31(4):255-61. doi: 10.1089/hyb.2012.0024.
Morbidity from allergic diseases is increasing. Basophils play a critical role in systemic anaphylaxis and chronic allergic inflammation. The prenatal environment must be regarded as a possible early risk factor for allergic diseases in children. Our objective was to determine if basophils harvested from neonates genetically predisposed to atopic disease had different levels of CD63 expression and IL-4 release properties in response to various stimuli (peptidoglycan, Dermatophagoides farinae, hyperosmotic mannitol). Blood samples were collected from 16 asthmatic and 18 healthy women and their newborns. Peripheral blood basophil histamine was measured using the human basophil degranulation test (HBDT), whereas activation was assessed by flow cytometric measurement of CD63 expression on the cord blood basophil surface. IL-4 levels were quantified by ELISA following allergen stimulation. The basophil degranulation index (DI) in granulocytes harvested from the peripheral blood of asthmatic women was assessed following stimulation with peptidoglycan (PGN), Dermatophagoides farinae (Df ) extract, or hyperosmotic mannitol. The DI was significantly higher in atopic women than in healthy controls. Upregulation of CD63 on the cord blood basophil surface was also detected in response to these stimuli. Basophils purified from the cord blood of neonates born to atopic mothers produced more IL-4 compared to basophils purified from the controls. These data suggested that various stimuli play a role in augmenting allergic reactions via modulation of activated basophil cytokine secretion. It may require new methods to stabilize the basophils in allergic diseases.
过敏性疾病的发病率正在上升。嗜碱性粒细胞在全身性过敏反应和慢性过敏性炎症中起关键作用。产前环境必须被视为儿童过敏性疾病可能的早期风险因素。我们的目标是确定从遗传易患特应性疾病的新生儿中采集的嗜碱性粒细胞在对各种刺激（肽聚糖、粉尘螨、高渗甘露醇）的反应中是否具有不同水平的CD63表达和IL-4释放特性。从16名哮喘女性和18名健康女性及其新生儿中采集血样。使用人嗜碱性粒细胞脱颗粒试验（HBDT）测量外周血嗜碱性粒细胞组胺，而通过流式细胞术测量脐血嗜碱性粒细胞表面CD63的表达来评估活化情况。在过敏原刺激后，通过ELISA对IL-4水平进行定量。在用肽聚糖（PGN）、粉尘螨（Df）提取物或高渗甘露醇刺激后，评估从哮喘女性外周血中采集的粒细胞中的嗜碱性粒细胞脱颗粒指数（DI）。特应性女性的DI显著高于健康对照组。在对这些刺激的反应中，还检测到脐血嗜碱性粒细胞表面CD63的上调。与从对照组纯化的嗜碱性粒细胞相比，从特应性母亲所生新生儿的脐血中纯化的嗜碱性粒细胞产生更多IL-4。这些数据表明，各种刺激通过调节活化的嗜碱性粒细胞细胞因子分泌在增强过敏反应中起作用。可能需要新的方法来稳定过敏性疾病中的嗜碱性粒细胞。
