Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Bani-Suef University, Alshaheed Shehata Ahmad Hegazy St., 62111 Beni-Suef, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Sep;113:291-6. doi: 10.1016/j.saa.2013.04.120. Epub 2013 May 21.
Accurate, selective and sensitive spectrophotometric methods have been developed and validated for simultaneous determination of Cinnarizine and Domperidone, a binary mixture with overlapping spectra, without preliminary separation. These methods include area under the curve (AUC) and dual wavelength spectrophotometry. For the AUC method, the area under curve of mixture solutions in the wavelength ranges 241-258 nm and 280-292 nm were selected for determination of Cinnarizine and Domperidone and by applying Cramer's rule, concentration of each drug was obtained. In dual wavelength method, two wavelengths were selected for each drug in a way so that the difference in absorbance is zero for another drug. Domperidone shows equal absorbance at 240.2 nm and 273.2 nm, where the differences in absorbance were measured for the determination of Cinnarizine. Similarly, differences in absorbance at 230.8 nm and 259.2 nm were measured for determination of Domperidone. The proposed methods were applied for determination of Cinnarizine and Domperidone over the concentration ranges of 2-20 and 2-22 μg mL(-1), respectively. The suggested methods were validated as per USP guidelines and the results revealed that they are reliable, reproducible and precise for routine use with short analysis time. The results obtained by the proposed methods were statistically compared to the reported method, and there was no significant difference between them regarding both accuracy and precision.
已经开发和验证了准确、选择性和灵敏的分光光度法，用于同时测定具有重叠光谱的二元混合物中的桂利嗪和多潘立酮，无需预先分离。这些方法包括曲线下面积（AUC）和双波长分光光度法。对于 AUC 方法，选择混合物溶液在 241-258nm 和 280-292nm 波长范围内的曲线下面积，用于测定桂利嗪和多潘立酮，并通过应用克拉默法则，获得每种药物的浓度。在双波长法中，选择每个药物的两个波长，使得另一种药物的吸光度差异为零。多潘立酮在 240.2nm 和 273.2nm 处具有相等的吸光度，用于测定桂利嗪时测量吸光度的差异。同样，在 230.8nm 和 259.2nm 处测量吸光度的差异，用于测定多潘立酮。所提出的方法适用于测定桂利嗪和多潘立酮的浓度范围分别为 2-20μg mL(-1)和 2-22μg mL(-1)。所提出的方法按照 USP 指南进行了验证，结果表明它们可靠、可重现且精确，可用于常规使用，分析时间短。所提出的方法的结果与报道的方法进行了统计学比较，在准确性和精密度方面没有显著差异。
