College of Animal Science and Technology, Yangzhou University, Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, Jiangsu 225009, PR China.
Gene. 2013 Sep 10;526(2):205-9. doi: 10.1016/j.gene.2013.05.030. Epub 2013 Jun 2.
Yangtze River Delta white goat is an exclusive indigenous Chinese goat breeds that can produce Brush Hair specialized in making valuable writing brush. The high-grade (Type III) Brush Hair is conical coarse hair but with a tip, which only grows in the cervical carina and back regions of Yangtze River Delta white goats. Screening of genes influencing the formation of Type III Brush Hair was conducted using differential display of mRNA technique in skin including the hair follicles of different goat groups and the differential bands were identified using reverse Northern dot blot, and the positive bands were subsequently cloned and sequenced. The results showed that 20 differentially displayed bands were obtained, seven of which were identified positive as expressed in skin. Three of these seven cDNAs were homologous to certain sequences from GenBank and the other four were respectively homologous to CKLF-like MARVEL transmembrane domain containing 3 (CMTM3), S100 calcium binding protein A4 (S100A4), protein kinase inhibitor gamma (PKIG) and fibulin 1-D. The study would provide a new view to elucidate their roles in hair growth and hair follicle cycle and increase our understanding of the formation of Type III Brush Hair.
长江三角洲白山羊是中国特有的山羊品种，其毛发可用于制作优质毛笔的羊毫。高档（三级）羊毫呈圆锥形粗毛，尖端有锋，仅生长在长江三角洲白山羊的颈嵴和背部。本研究采用 mRNA 差异显示技术，对不同群体山羊的皮肤（包括毛囊）进行基因筛选，通过反转 Northern 点杂交鉴定差异表达条带，然后对阳性条带进行克隆和测序。结果共获得 20 条差异表达条带，其中 7 条在皮肤中表达。这 7 个 cDNA 中有 3 个与 GenBank 中的某些序列同源，其余 4 个分别与 CKLF 样 MARVEL 跨膜结构域包含蛋白 3（CMTM3）、S100 钙结合蛋白 A4（S100A4）、蛋白激酶抑制剂 γ（PKIG）和纤维蛋白 1-D 同源。本研究为阐明它们在毛发生长和毛囊周期中的作用提供了新的视角，也增加了我们对三级羊毫形成的理解。
