College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
In Vitro Cell Dev Biol Anim. 2021 Apr;57(4):428-437. doi: 10.1007/s11626-021-00557-7. Epub 2021 Mar 21.
The Yangtze River Delta White Goat is the only goat breed in the world that can produce superior-quality brush hair. Previous studies have shown that some genes are expressed differentially in the skin tissues between the goats produced superior-quality and normal-quality brush hair. Studies also have shown that different gene play varied roles in regulating the proliferation and apoptosis of hair follicle stem cells. However, the biological function of MAP3K1 (mitogen-activated protein kinase kinase kinase 1) gene in hair follicle stem cells is not fully understood. This study aims to investigate the role of MAP3K1 knockdown during the proliferation and apoptosis of hair follicle stem cells. RT-qPCR and Western blot were used to detect mRNA gene and protein expression level, CCK-8 and EdU assays were used to detect cell proliferation, and cell cycle and apoptosis were detected by flow cytometry. The results showed that the MAP3K1 expression level was significantly higher in the skin tissue of produced superior-quality brush hair than that in produced normal-quality brush hair. Moreover, functional studies indicated that si-MAP3K1 significantly inhibits the proliferation of hair follicle stem cells that came from a superior goat and promotes its apoptosis. Based on aforementioned assays, we speculated that MAP3K1 might play a regulatory effect in superior-quality brush hair traits.
长江三角洲白山羊是世界上唯一能生产优质毛笔毛的山羊品种。先前的研究表明，在产优质笔毛和普通笔毛的山羊皮肤组织中，一些基因的表达存在差异。研究还表明，不同的基因在调节毛囊干细胞的增殖和凋亡中发挥不同的作用。然而，MAP3K1（丝裂原活化蛋白激酶激酶激酶 1）基因在毛囊干细胞中的生物学功能尚不完全清楚。本研究旨在探讨 MAP3K1 敲低在毛囊干细胞增殖和凋亡中的作用。实时荧光定量 PCR 和 Western blot 用于检测 mRNA 基因和蛋白表达水平，CCK-8 和 EdU 检测用于检测细胞增殖，流式细胞术用于检测细胞周期和凋亡。结果表明，在产优质笔毛的山羊皮肤组织中，MAP3K1 的表达水平明显高于产普通笔毛的山羊。此外，功能研究表明，si-MAP3K1 显著抑制了来自优质山羊的毛囊干细胞的增殖，并促进其凋亡。基于上述检测，我们推测 MAP3K1 可能在优质笔毛性状中发挥调节作用。
