Particulate Fluids Processing Centre, School of Chemistry, University of Melbourne, Parkville, Victoria 3010, Australia.
Ultrason Sonochem. 2014 Jan;21(1):23-8. doi: 10.1016/j.ultsonch.2013.05.004. Epub 2013 May 18.
Ultrasonic technique has been used for synthesising protein microspheres possessing specific physical and functional properties. Various proteins have been used as shell materials under different experimental conditions. In previous studies, thermal or chemical denaturation of the proteins was used to obtain stable bovine-serum albumin (BSA) and lysozyme microbubbles (MBs), respectively. It is ideal to establish a generic procedure to synthesise microspheres irrespective of the nature of the protein. In order to see if a generic procedure can be established, ultrasonic synthesis of lysozyme and BSA MBs was carried out under similar experimental conditions and their properties were evaluated. The size, size distribution and the stability of the MBs were significantly different for the lysozyme and BSA MBs. The size and size distribution of the lysozyme coated MBs were larger than BSA bubbles. The mechanical strength of MBs against the shear forces, generated when irradiated by high frequency ultrasound, was studied using pulsed-sonoluminescence (SL). This study indicated that lysozyme MBs were significantly more stable than BSA MBs. An increase in mechanical strength of the MBs may lead to an increase in their storage lifetime and stability against gas diffusion. Possible reasons for such observations have been discussed.
超声技术已被用于合成具有特定物理和功能特性的蛋白质微球。在不同的实验条件下，已经使用了各种蛋白质作为壳材料。在以前的研究中，分别使用热或化学变性来获得稳定的牛血清白蛋白（BSA）和溶菌酶微泡（MB）。理想的情况是建立一种通用的方法来合成微球，而不考虑蛋白质的性质。为了验证是否可以建立一种通用的方法，在相似的实验条件下进行了溶菌酶和 BSA MB 的超声合成，并对其性质进行了评估。溶菌酶和 BSA MB 的大小、大小分布和稳定性有显著差异。溶菌酶包被的 MB 的大小和大小分布大于 BSA 气泡。使用脉冲声致发光（SL）研究了高频超声辐照时产生的剪切力对 MB 的机械强度的影响。这项研究表明，溶菌酶 MB 比 BSA MB 稳定得多。MB 机械强度的增加可能会导致其储存寿命和对气体扩散的稳定性增加。对这些观察结果的可能原因进行了讨论。
