Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan.
Department of Biomedical Engineering, National Defense Medical Center, Taipei 11490, Taiwan.
Sci Rep. 2017 Jan 24;7:41325. doi: 10.1038/srep41325.
Acne vulgaris is the most common skin disorder, and is caused by Propionibacterium acnes (P. acnes) and can induce inflammation. Antibiotic therapy often needs to be administered for long durations in acne therapy, which results in extensive antibiotic exposure. The present study investigated a new treatment model for evaluating the antibacterial effects of lysozyme (LY)-shelled microbubbles (MBs) and ultrasound (US)-mediated LY-shelled MBs cavitation against P. acnes both in vitro and in vivo, with the aims of reducing the dose and treatment duration and improving the prognosis of acne vulgaris. In terms of the in vitro treatment efficacy, the growth of P. acnes was inhibited by 86.08 ± 2.99% in the LY-shelled MBs group and by 57.74 ± 3.09% in the LY solution group. For US power densities of 1, 2, and 3 W/cm in the LY-shelled MBs group, the growth of P. acnes was inhibited by 95.79 ± 3.30%, 97.99 ± 1.16%, and 98.69 ± 1.13%, respectively. The in vivo results showed that the recovery rate on day 13 was higher in the US group with LY-shelled MBs (97.8 ± 19.8%) than in the LY-shelled MBs group (90.3 ± 23.3%). Our results show that combined treatments of US and LY-shelled MBs can significantly reduce the treatment duration and inhibit P.-acnes-induced inflammatory skin diseases.
寻常痤疮是最常见的皮肤疾病，由痤疮丙酸杆菌（P. acnes）引起，并可诱发炎症。痤疮的治疗通常需要长期使用抗生素，这会导致广泛的抗生素暴露。本研究旨在评估溶菌酶（LY）壳微泡（MBs）和超声（US）介导的 LY 壳 MBs 空化对 P. acnes 的体外和体内抗菌作用，探索一种新的治疗模型，以减少剂量和治疗时间，并改善寻常痤疮的预后。在体外治疗效果方面，LY 壳 MBs 组中 P. acnes 的生长抑制率为 86.08±2.99%，LY 溶液组中为 57.74±3.09%。在 LY 壳 MBs 组中，US 功率密度为 1、2 和 3 W/cm 时，P. acnes 的生长抑制率分别为 95.79±3.30%、97.99±1.16%和 98.69±1.13%。体内结果表明，US 联合 LY 壳 MBs 治疗组第 13 天的恢复率（97.8±19.8%）高于 LY 壳 MBs 组（90.3±23.3%）。我们的结果表明，US 和 LY 壳 MBs 的联合治疗可以显著缩短治疗时间并抑制 P.-acnes 引起的炎症性皮肤疾病。
