Shiba Y, Kanno Y
Department of Oral Physiology, Hiroshima University School of Dentistry, Japan.
Hiroshima J Med Sci. 1990 Mar;39(1):11-4.
To clarify the energy-dependency of biliary excretion of a diagnostic dye, the effect of ATP depletion on biliary excretion was investigated. Biliary excretion of indocyanine green (ICG) in rat liver reached a maximum 20 min after intravenous application. The level of ATP in the liver was lowered to 35.4% of the control value about 5 h after administration of DL-ethionine (100 mg/100 g body weight). The rate of bile flow and the amount of ICG excreted in initial 10 min into the bile of ethionine-treated rats were lowered to 67.5% and 61.3% of the control values, respectively. The amount of ICG excreted for 10 min from 30 min to 40 min after application of ICG in ethionine-treated rats was comparable to that of the control. The level of ICG in blood 5 min after application of ICG was raised by 59.2% of the control value. These results suggest that ATP depletion in the liver caused by treatment with ethionine suppresses the initial rate of uptake and biliary excretion of ICG.
为阐明诊断用染料经胆汁排泄的能量依赖性，研究了ATP耗竭对胆汁排泄的影响。静脉注射后20分钟，大鼠肝脏中吲哚菁绿（ICG）的胆汁排泄量达到最大值。给予DL-乙硫氨酸（100mg/100g体重）约5小时后，肝脏中的ATP水平降至对照值的35.4%。乙硫氨酸处理的大鼠胆汁流量速率以及最初10分钟内排入胆汁中的ICG量分别降至对照值的67.5%和61.3%。在乙硫氨酸处理的大鼠中，注射ICG后30分钟至40分钟期间10分钟内排泄的ICG量与对照相当。注射ICG 5分钟后血液中ICG水平比对照值升高了59.2%。这些结果表明，乙硫氨酸处理导致的肝脏ATP耗竭会抑制ICG的初始摄取速率和胆汁排泄。
