Barth A, Gregus Z, Klinger W, Varga F
Acta Biol Med Ger. 1981;40(12):1723-8.
In rats of different ages bile flow and biliary excretion of indocyanine green (ICG) were estimated in dependence on bile acid excretion. In 20, 30, 60 and 180 day old rats bile acid excretion was diminished to a quarter of the control by pretreatment with cholestyramine (1.5 g/kg b.wt.). To enhance bile acid excretion, taurocholate sodium (TC, 50 mg/kg b.wt.) was given together with the dye. Bile acid depletion diminished the bile flow to a greater extent in young rats, which were found to have a higher bile acid dependent fraction of canalicular bile flow (60%) compared to 60 (35%) and 180 day old rats (20%). After bile acid depletion the decrease in ICG excretion was more pronounced in 30 and 60 day old rats which exhibit highest dye excretion rates. TC was able to enhance the bile flow in all age groups investigated, however, the ICG excretion was enhanced only in adult animals. Our results indicate that the bile acid dependence of the hepatobiliary transport of ICG is influenced by age.
在不同年龄的大鼠中，根据胆汁酸排泄情况对胆汁流量和吲哚菁绿（ICG）的胆汁排泄进行了评估。通过用消胆胺（1.5 g/kg体重）预处理，20日龄、30日龄、60日龄和180日龄大鼠的胆汁酸排泄减少至对照的四分之一。为了增加胆汁酸排泄，将牛磺胆酸钠（TC，50 mg/kg体重）与染料一起给予。胆汁酸耗竭在幼鼠中更大程度地减少了胆汁流量，发现幼鼠胆小管胆汁流量中胆汁酸依赖性部分（60%）高于60日龄（35%）和180日龄大鼠（20%）。胆汁酸耗竭后，ICG排泄的减少在染料排泄率最高的30日龄和60日龄大鼠中更为明显。TC能够增加所有研究年龄组的胆汁流量，然而，仅在成年动物中ICG排泄增加。我们的结果表明，ICG肝胆转运的胆汁酸依赖性受年龄影响。
