Kapfenberger David, Sonn-Segev Adar, Roichman Yael
School of Physics, Tel Aviv University, Tel Aviv 69978, Israel.
Opt Express. 2013 May 20;21(10):12228-37. doi: 10.1364/OE.21.012228.
In-line holographic optical imaging has the unique capability of high speed imaging in three dimensions at rates limited only by the imaging rate of the camera used. In this technique the 3D data is recorded on the detector in a form of a hologram generated by diffraction between the scattered and unscattered light passing through the sample. For dilute samples of single particles or a small cluster of particles, this technique was shown to result in particle tracking with spatial positioning accuracy of a few nanometers. For dense suspension only approximate reconstruction were achieved with systematic axial positioning errors. We propose a scheme to extend accurate holographic microscopy to dense suspensions, by calibrating the Rayleigh-Sommerfeld reconstruction algorithm against Lorentz-Mie scattering theory. We perform this calibration both numerically and experimentally and define the parameter space in which accurate imaging is achieved, and in which numerical calibration holds. We demonstrate the validity of our approach by imaging two attached particles and measuring the distance between their centers with 36 nm accuracy. A difference of 50 nm in particle diameter is easily measured.
在线全息光学成像具有独特的能力，能够以仅受所用相机成像速率限制的速度进行三维高速成像。在这种技术中，三维数据以全息图的形式记录在探测器上，该全息图由穿过样品的散射光和非散射光之间的衍射产生。对于单个粒子或一小簇粒子的稀溶液，该技术已被证明能实现空间定位精度达几纳米的粒子跟踪。对于密集悬浮液，仅实现了具有系统轴向定位误差的近似重建。我们提出了一种方案，通过根据洛伦兹 - 米氏散射理论校准瑞利 - 索末菲重建算法，将精确的全息显微镜扩展到密集悬浮液。我们通过数值和实验两种方式进行这种校准，并定义实现精确成像以及数值校准有效的参数空间。我们通过对两个附着粒子成像并以36纳米的精度测量它们中心之间的距离，证明了我们方法的有效性。粒子直径50纳米的差异很容易测量出来。
