Cheong Fook Chiong, Krishnatreya Bhaskar Jyoti, Grier David G
Department of Physics and Center for Soft Matter Research, New York University, New York, NY 10003, USA.
Opt Express. 2010 Jun 21;18(13):13563-73. doi: 10.1364/OE.18.013563.
The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution. In this work, we compare the performance of two complementary analysis techniques, one based on fitting to the exact Lorenz-Mie theory and the other based on phenomenological interpretation of the scattered light field reconstructed with Rayleigh-Sommerfeld back-propagation. Although Lorenz-Mie tracking provides more information and is inherently more precise, Rayleigh-Sommerfeld reconstruction is faster and more general. The two techniques agree quantitatively on colloidal spheres' in-plane positions. Their systematic differences in axial tracking can be explained in terms of the illuminated objects' light scattering properties.
在线全息显微镜捕获的视频流可以逐帧分析，以纳米分辨率跟踪单个胶体颗粒的三维运动。在这项工作中，我们比较了两种互补分析技术的性能，一种基于对精确洛伦兹-米氏理论的拟合，另一种基于用瑞利-索末菲反向传播重建的散射光场的唯象解释。虽然洛伦兹-米氏跟踪提供了更多信息且本质上更精确，但瑞利-索末菲重建更快且更通用。这两种技术在胶体球的平面内位置上定量一致。它们在轴向跟踪上的系统差异可以根据被照射物体的光散射特性来解释。
