State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-environmental Science, Chinese Academy of Sciences, Beijing 100085, China.
Sci China Life Sci. 2013 Jun;56(6):561-70. doi: 10.1007/s11427-013-4486-0. Epub 2013 Jun 5.
This study investigated the influence of broadleaf and conifer vegetation on soil microbial communities in a distinct vertical distribution belt in Northeast China. Soil samples were taken at 0-5, 5-10 and 10-20 cm depths from four vegetation types at different altitudes, which were characterized by poplar (Populus davidiana) (1250-1300 m), poplar (P. davidiana) mixed with birch (Betula platyphylla) (1370-1550 m), birch (B. platyphylla) (1550-1720 m), and larch (Larix principis-rupprechtii) (1840-1890 m). Microbial biomass and community structure were determined using the fumigation-extraction method and phospholipid fatty acid (PLFA) analysis, and soil fungal community level physiological profiles (CLPP) were characterized using Biolog FF Microplates. It was found that soil properties, especially soil organic carbon and water content, contributed significantly to the variations in soil microbes. With increasing soil depth, the soil microbial biomass, fungal biomass, and fungal catabolic ability diminished; however, the ratio of fungi to bacteria increased. The fungal ratio was higher under larch forests compared to that under poplar, birch, and their mixed forests, although the soil microbial biomass was lower. The direct contribution of vegetation types to the soil microbial community variation was 12%. If the indirect contribution through soil organic carbon was included, variations in the vegetation type had substantial influences on soil microbial composition and diversity.
本研究调查了阔叶和针叶植被对中国东北地区一个独特垂直分布带土壤微生物群落的影响。从四个不同海拔高度的植被类型中采集了 0-5、5-10 和 10-20 厘米深度的土壤样本，这些植被类型分别为杨树（Populus davidiana）（1250-1300 米）、杨桦混交林（1370-1550 米）、白桦林（1550-1720 米）和落叶松林（1840-1890 米）。采用熏蒸提取法和磷脂脂肪酸（PLFA）分析测定微生物生物量和群落结构，并用 Biolog FF 微孔板测定土壤真菌群落水平生理图谱（CLPP）。结果表明，土壤性质，特别是土壤有机碳和含水量，对土壤微生物的变化有显著影响。随着土壤深度的增加，土壤微生物生物量、真菌生物量和真菌代谢能力下降，而真菌与细菌的比例增加。落叶松林下的真菌比例高于杨桦林及其混交林，尽管土壤微生物生物量较低。植被类型对土壤微生物群落变化的直接贡献为 12%。如果包括通过土壤有机碳的间接贡献，植被类型的变化对土壤微生物组成和多样性有很大的影响。
