Kuhn Katharina, Rudolph Heike, Luthardt Ralph G, Stock Karl, Diebolder Rolf, Hibst Raimund
Center of Dentistry, Department of Prosthetic Dentistry, Ulm University, 89081, Ulm, Germany.
Lasers Surg Med. 2013 Jul;45(5):339-44. doi: 10.1002/lsm.22143. Epub 2013 Jun 4.
The aim of this in vitro study was to investigate the effect of Er:YAG laser irradiation on the ability of sodium hypochlorite (NaOCl) to dissolve soft tissue during endodontic procedures.
Two acrylic glass plates, each containing a semi-canal, were bolted together to form a complete canal. This geometry permitted one semi-canal to be filled with fine liver sausage of bovine origin dyed by methylene blue and the other with NaOCl (4.00-4.99% available chlorine; Sigma-Aldrich Corporation, St. Louis, MA), which was then activated by Er:YAG laser irradiation (KEY Laser 3; KaVo, Biberach, Germany) using a plain-ended fiber tip and a range of output energy and repetition rate. To achieve relatively low output energy from high input energy, the laser beam was attenuated by placing glass slides in the beam path. The resultant images acquired were analyzed using pixel-based analysis. Samples were statistically analyzed (two-way ANOVA, P < 0.05, univariate, bifactorial; IBM SPSS Statistics 19, SPSS Inc., Chicago, IL).
Both output energy and repetition rate significantly influenced the tissue dissolution ability of NaOCl (P < 0.05).
Within the limitations of this in vitro study, we conclude that laser activation of NaOCl at 200 mW output power leads to effective soft tissue dissolution. This finding can be of use to endodontists pursuing effective soft tissue dissolution from their irrigants.
本体外研究旨在调查铒激光照射对次氯酸钠（NaOCl）在根管治疗过程中溶解软组织能力的影响。
将两块各含一个半根管的丙烯酸玻璃板用螺栓固定在一起形成一个完整的根管。这种结构允许一个半根管填充用亚甲蓝染色的牛源精细肝组织，另一个填充NaOCl（有效氯含量为4.00 - 4.99%；西格玛奥德里奇公司，密苏里州圣路易斯），然后使用平端光纤头和一系列输出能量及重复频率，通过铒激光照射（KEY Laser 3；德国卡瓦公司，比伯拉赫）对其进行激活。为了从高输入能量获得相对较低的输出能量，通过在光路中放置载玻片来衰减激光束。使用基于像素的分析方法对获取的图像进行分析。对样本进行统计学分析（双向方差分析，P < 0.05，单变量，双因素；IBM SPSS Statistics 19，SPSS公司，伊利诺伊州芝加哥）。
输出能量和重复频率均显著影响NaOCl的组织溶解能力（P < 0.05）。
在本体外研究的局限性范围内，我们得出结论，输出功率为200 mW时，激光激活NaOCl可有效溶解软组织。这一发现对于寻求从冲洗剂中有效溶解软组织的牙髓病医生可能有用。
