Juárez-García Erika, Agama-Acevedo Edith, Gómez-Montiel Noel Orlando, Pando-Robles Victoria, Bello-Pérez Luis Arturo
Instituto Politécnico Nacional, CEPROBI, colonia San Isidro, 62731, Yautepec, Morelos, México.
J Sci Food Agric. 2013 Aug 30;93(11):2660-8. doi: 10.1002/jsfa.6054. Epub 2013 Jun 4.
The characterization of starch maize with different endosperm type and the proteomic analysis of its biosynthetic enzymes at 20 and 50 days after pollination (DAP) was carried out.
There were differences between both endosperm types at 20 DAP, mainly in starch accumulation, amylose content, granule size and crystallinity percentage, whereas at 50 DAP the differences found were not relevant in the case of starch content, granule size, chain length distribution and thermal properties. SSSI, SBEIIb and GBSSI enzymes were identified; however, SBEIIb was only identified in two samples: floury endosperm at 20 DAP and vitreous at 50 DAP.
Starch did not show differences in its morphological or structural characteristics in either endosperm on reaching maturity. Starch biosynthetic enzymes identified by matrix-assisted laser desorption/ionization-time of flight did not show a relationship to starch structure.
对不同胚乳类型的粉质玉米进行了表征，并在授粉后20天和50天对其生物合成酶进行了蛋白质组学分析。
在授粉后20天，两种胚乳类型之间存在差异，主要体现在淀粉积累、直链淀粉含量、颗粒大小和结晶度百分比方面；而在授粉后50天，在淀粉含量、颗粒大小、链长分布和热性质方面发现的差异并不显著。已鉴定出SSSI、SBEIIb和GBSSI酶；然而，SBEIIb仅在两个样品中被鉴定出来：授粉后20天的粉质胚乳和授粉后50天的玻璃质胚乳。
成熟时，两种胚乳中的淀粉在形态或结构特征上均未表现出差异。通过基质辅助激光解吸/电离飞行时间鉴定出的淀粉生物合成酶与淀粉结构无关。
