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用共聚焦激光扫描显微镜揭示的果蝇胚胎细胞化过程中的F-肌动蛋白组织。

F-actin organization during the cellularization of the Drosophila embryo as revealed with a confocal laser scanning microscope.

作者信息

Warn R M, Robert-Nicoud M

机构信息

School of Biological Sciences, University of East Anglia, Norwich, UK.

出版信息

J Cell Sci. 1990 May;96 ( Pt 1):35-42. doi: 10.1242/jcs.96.1.35.

DOI:10.1242/jcs.96.1.35
PMID:2373743
Abstract

The changes in F-actin organization during the cellularization of the Drosophila embryo have been studied with a confocal laser scanning microscope using fluorescein-phalloidin as a specific stain. Particular study has been made of the changes in the organization of the F-actin network associated with the leading edges of the growing membranes. The role of this actin network in the cellularization process is considered. Other actin-containing structures have also been examined, including the cortical actin layer and a conspicuous region of F-actin aggregates, present beneath the level of the forming cell membranes.

摘要

利用荧光素-鬼笔环肽作为特异性染色剂,通过共聚焦激光扫描显微镜研究了果蝇胚胎细胞化过程中F-肌动蛋白组织的变化。特别研究了与生长膜前缘相关的F-肌动蛋白网络组织的变化。考虑了该肌动蛋白网络在细胞化过程中的作用。还检查了其他含肌动蛋白的结构,包括皮质肌动蛋白层和在形成的细胞膜水平以下存在的明显的F-肌动蛋白聚集体区域。

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