Riparbelli M G, Dallai R, Callaini G
Department of Evolutionary Biology, University of Siena, Italy.
Biol Cell. 1993;78(3):223-7. doi: 10.1016/0248-4900(93)90133-y.
Rhodamine phalloidin-staining was used to study the organization of the cortical actin cytoskeleton of the early Ceratitis capitata embryo. The dynamics of the actin aggregates and their changes in distribution during the formation of the syncytial blastoderm, were followed in detail. It was found that these aggregates formed a shell-like cluster around the interphase nuclei, and concentrated toward the poles of the mitotic apparatus when the nuclei divided. Laser scanning confocal microscopy revealed that aggregates not clustered at the poles of the mitotic apparatus were closely associated with fine fibers of a dense cytoplasmic network of actin filaments.
使用罗丹明鬼笔环肽染色来研究早期地中海实蝇胚胎皮质肌动蛋白细胞骨架的组织。详细追踪了肌动蛋白聚集体的动态变化及其在合胞体胚盘形成过程中的分布变化。发现这些聚集体在间期核周围形成壳状簇，并在核分裂时向有丝分裂器的两极集中。激光扫描共聚焦显微镜显示，未聚集在有丝分裂器两极的聚集体与肌动蛋白丝致密细胞质网络的细纤维紧密相关。
