Arthritis Research Laboratory, Hanson Institute, SA Pathology, Adelaide, SA 5000, Australia.
Mediators Inflamm. 2013;2013:329494. doi: 10.1155/2013/329494. Epub 2013 May 7.
Prostaglandin (PG)D2 has been shown to be an active agent in the resolution of experimentally induced inflammation. This study was undertaken to determine the presence of PGD2 in chronic joint effusions and to explore the potential contributions of dendritic cells (DC) and monocytes to the intra-articular synthesis of PGD2. Synovial fluid (SF) was obtained from patients with inflammatory arthritis and knee effusions. PGD2 and PGE2 were detected in SF by ultrahigh-performance tandem mass spectrometry. Cellular fractions in SF were separated by density-gradient centrifugation and flow cytometry. The expression of hematopoietic prostaglandin D-synthase (hPGDS) and PGE-synthase (PGES) mRNA was determined by RT-PCR. Both PGD2 and PGE2 were detected in blood and SF, with PGD2 being more abundant than PGE2 in SF. mRNA for hPGDS was more abundant in SF mDCs than SF monocytes (P < 0.01) or PB monocytes (P < 0.001). SF mDC expressed significantly more hPGDS than PGES. Expressions of PGD2 and hPGDS were inversely associated with serum C-reactive protein (P < 0.01) and erythrocyte sedimentation rate (P < 0.01). The findings suggest that synovial DCs may be an important source of hPGDS and that systemic disease activity may be influenced by actions of PGD2 in RA and other arthropathies.
前列腺素 (PG)D2 已被证明是实验性诱导炎症消退的有效因子。本研究旨在确定慢性关节积液中是否存在 PGD2，并探讨树突状细胞 (DC) 和单核细胞对关节内 PGD2 合成的潜在贡献。从患有炎症性关节炎和膝关节积液的患者中获得滑液 (SF)。通过超高效串联质谱法检测 SF 中的 PGD2 和 PGE2。通过密度梯度离心和流式细胞术分离 SF 中的细胞成分。通过 RT-PCR 确定 SF 中造血前列腺素 D-合酶 (hPGDS) 和 PGE-合酶 (PGES) mRNA 的表达。在血液和 SF 中均检测到 PGD2 和 PGE2，SF 中 PGD2 比 PGE2 更丰富。SF mDC 中的 hPGDS mRNA 比 SF 单核细胞 (P < 0.01) 或 PB 单核细胞 (P < 0.001) 更丰富。SF mDC 表达的 hPGDS 明显多于 PGES。PGD2 和 hPGDS 的表达与血清 C 反应蛋白 (P < 0.01) 和红细胞沉降率 (P < 0.01) 呈负相关。这些发现表明滑膜 DC 可能是 hPGDS 的重要来源，全身疾病活动可能受到 RA 和其他关节病中 PGD2 作用的影响。
