Department of Rheumatology, Catholic University of the Sacred Heart, CIC, Via Moscati 31, 00168, Rome, Italy.
Clin Immunol. 2011 Jan;138(1):107-16. doi: 10.1016/j.clim.2010.10.002. Epub 2010 Nov 5.
We analyzed peripheral blood (PB) and synovial fluid (SF) mononuclear cells from 16 rheumatoid arthritis (RA), 9 spondyloarthritis (SpA), 3 microcrystal arthritis patients, to define the presence of Th17 and Th1 and their relationship with inflammatory activity, and TCR-zeta chain and ZAP-70 levels. Th17 were significantly higher in SF than in PB and more abundant in microcrystal arthritis patients compared to the other groups. Irrespectively of the diagnosis, SF Th17 percentages correlated with joint (SF total leukocyte count, neutrophil percentage) and systemic (C reactive protein [CRP], fibrinogen, erythrocyte sedimentation rate) inflammation markers. SF Th1 percentages directly correlated with inflammation and disease activity (CRP, swollen joint count [SJC]) indices in SpA, but not in RA patients. These observations support the role of Th17 in the pathogenesis of inflammatory arthritides. The TCR-zeta(dim) lymphocytes in SF were found to produce the highest amounts of cytokines including IL-17, whereas no ZAP-70 impairment was associated to Th17.
我们分析了 16 例类风湿关节炎（RA）、9 例脊柱关节炎（SpA）和 3 例微晶体关节炎患者的外周血（PB）和滑膜液（SF）单核细胞，以确定 Th17 和 Th1 的存在及其与炎症活动的关系，以及 TCR-ζ 链和 ZAP-70 水平。SF 中的 Th17 明显高于 PB，并且在微晶体关节炎患者中比其他组更为丰富。无论诊断如何，SF Th17 百分比与关节（SF 总白细胞计数、中性粒细胞百分比）和全身（C 反应蛋白[CRP]、纤维蛋白原、红细胞沉降率）炎症标志物相关。SF Th1 百分比与 SpA 患者的炎症和疾病活动（CRP、肿胀关节计数[SJC]）指数直接相关，但在 RA 患者中则不相关。这些观察结果支持 Th17 在炎症性关节炎发病机制中的作用。在 SF 中发现 TCR-ζ（dim）淋巴细胞产生包括 IL-17 在内的最高量细胞因子，而 Th17 则与 ZAP-70 损伤无关。
