Petyaev Ivan M, Zigangirova Naylia A, Kobets Natalie V, Tsibezov Valery, Kapotina Lydia N, Fedina Elena D, Bashmakov Yuriy K
Lycotec Ltd. Granta Park Campus, Cambridge, UK.
ScientificWorldJournal. 2013 Apr 28;2013:140591. doi: 10.1155/2013/140591. Print 2013.
Inflammation in atherosclerosis, which could be associated with some subclinical infections such as C. pneumoniae, is one of the key factors responsible for the development of clinical complications of this disease. We report that a proprietary protein extract isolated from Roquefort cheese inhibits the propagation of C. pneumoniae in a human HL cell line in a dose-dependent manner, as revealed by the immunofluorescence analysis. These changes were accompanied by a significant reduction in the infective progeny formation over the protein extract range of 0.12-0.5 μg/mL. Moreover, short term feeding of mice with Roquefort cheese (twice, 10 mg per mouse with an interval of 24 hours) led to the inhibition of the migration of peritoneal leukocytes caused by intraperitoneal injection of E. coli lipopolysaccharide. These changes were complemented by a reduction in neutrophil count and a relative increase in peritoneal macrophages, suggesting that ingestion of Roquefort could promote regenerative processes at the site of inflammation. The ability of this protein to inhibit propagation of Chlamydia infection, as well as the anti-inflammatory and proregenerative effects of Roquefort itself, may contribute to the low prevalence of cardiovascular mortality in France where consumption of fungal fermented cheeses is the highest in the world.
动脉粥样硬化中的炎症可能与某些亚临床感染如肺炎衣原体有关，是该疾病临床并发症发生的关键因素之一。我们报告，从罗克福尔干酪中分离出的一种专利蛋白提取物，通过免疫荧光分析显示，它以剂量依赖的方式抑制肺炎衣原体在人HL细胞系中的繁殖。在0.12 - 0.5μg/mL的蛋白提取物范围内，这些变化伴随着感染性子代形成的显著减少。此外，用罗克福尔干酪对小鼠进行短期喂食（两次，每只小鼠10mg，间隔24小时）导致腹腔注射大肠杆菌脂多糖引起的腹腔白细胞迁移受到抑制。这些变化伴随着中性粒细胞计数的减少和腹腔巨噬细胞的相对增加，表明食用罗克福尔干酪可以促进炎症部位的再生过程。这种蛋白抑制衣原体感染传播的能力，以及罗克福尔干酪本身的抗炎和促再生作用，可能是法国心血管死亡率低的原因之一，法国是世界上食用真菌发酵干酪最多的国家。
