Lin Fan, Cao Jie, Huang Zhiming, Pei Zhenghao, Gu Weili, Fan Shaofeng, Li Kunping, Weng Jiefeng
Department of General Surgery, The First People's Hospital Affiliated to Guangzhou Medical University, Guangzhou, Guangdong 510180;
Exp Ther Med. 2013 May;5(5):1403-1407. doi: 10.3892/etm.2013.1010. Epub 2013 Mar 15.
The aim of the present study was to investigate the inhibitory effects of thalidomide in the hepatocellular carcinoma nude mouse model in order to provide new insights into a comprehensive clinical intervention for hepatocellular carcinoma. MHCC97 cells were routinely cultured, passaged and adjusted to a single cell suspension with a concentration of 2×10/ml. Six-week-old, BALB/C male nude mice were anesthetized and fixed in the prone position, then a subcapsular injection of the single cell suspension was administered into the spleen and their abdomens were closed. A laparotomy and left hepatic lobectomy was performed 14 days later and the abdomens were closed once again. Subsequent to the establishment of the hepatocellular carcinoma model, the nude mice were randomly divided into three groups, each consisting of 12 mice. The early intervention group were immediately provided with the post-operative thalidomide intervention, the late intervention group were provided with the post-operative thalidomide intervention one week subsequent to the surgery, and the negative control group were provided with a placebo intervention (0.9% physiological saline). Each intervention was continuously administered once per day for one week. The osteopontin (OPN) content of the liver tumors was detected using immunohistochemistry. The data were analyzed using an analysis of variance (ANOVA) test. There were significant differences in the OPN levels of the tumors among the early intervention, late intervention and negative control groups. Thalidomide may inhibit the generation of OPN and thereby inhibit the infiltration and metastasis of tumors; the immediate use of thalidomide following hepatectomy in the present study may block the invasion and metasis for liver cancer more effectively.
本研究的目的是探讨沙利度胺对肝癌裸鼠模型的抑制作用，以便为肝癌的综合临床干预提供新的见解。常规培养MHCC97细胞，传代并调整为浓度为2×10/ml的单细胞悬液。将6周龄的BALB/C雄性裸鼠麻醉并固定为俯卧位，然后将单细胞悬液经脾包膜下注射，缝合腹部。14天后进行剖腹手术和左肝叶切除术，再次缝合腹部。在建立肝癌模型后，将裸鼠随机分为三组，每组12只。早期干预组术后立即给予沙利度胺干预，晚期干预组在手术后一周给予沙利度胺干预，阴性对照组给予安慰剂干预（0.9%生理盐水）。每种干预每天持续给药一次，共一周。采用免疫组织化学法检测肝肿瘤中骨桥蛋白（OPN）的含量。数据采用方差分析（ANOVA）检验进行分析。早期干预组、晚期干预组和阴性对照组肿瘤的OPN水平存在显著差异。沙利度胺可能抑制OPN的产生，从而抑制肿瘤的浸润和转移；在本研究中，肝切除术后立即使用沙利度胺可能更有效地阻断肝癌的侵袭和转移。
