Department of Hepatobiliary Surgery, First Municipal People's Hospital of Guangzhou, The Affiliated Hospital of Guangzhou Medical College, Guangzhou 510180, People's Republic of China.
Mol Biol Rep. 2011 Nov;38(8):5205-10. doi: 10.1007/s11033-010-0671-4. Epub 2010 Dec 29.
Osteopontin (OPN) plays an important role in metastasis and relapse of human cancer. However, the whole story of OPN relating to cancer has been far from clear untill now. To investigate the expression of OPN in hepatocellular carcinoma (HCC) and its relationships with recurrence and metastasis of HCC, normal and malignant liver tissues from patients with HCC were analyzed using immunohistochemical staining. OPN expression was inhibited by small interfering RNA (siRNA) in HCC cells lines, and then colony formation and matrigel invasion were examined. The results showed that expression of OPN was associated with metastasis of HCC with a positive rate of OPN in the tissue of HCC (70.00%), which was highly more obvious than those in paracarcinoma tissue and normal liver tissue (P < 0.01). In HCC cell lines, OPN depletion could reduce formed colony and metastasizing numbers in vitro. In conclusion, Expression of OPN in the tissue of HCC is related to metastasis or metastases. Specific siRNA could decrease expressions of OPN at both mRNA and protein levels, and abates the invasiveness of hepatocellular carcinoma cells, suggesting that OPN might be a promising agent for treatment of metastasis and recurrence of HCC.
骨桥蛋白（OPN）在人类癌症的转移和复发中起着重要作用。然而，直到现在，OPN 与癌症相关的全部情况还远未清楚。为了研究骨桥蛋白在肝细胞癌（HCC）中的表达及其与 HCC 复发和转移的关系，采用免疫组织化学染色分析了 HCC 患者的正常和恶性肝组织中的 OPN 表达。用小干扰 RNA（siRNA）抑制 HCC 细胞系中的 OPN 表达，然后检查集落形成和基质胶侵袭。结果表明，OPN 的表达与 HCC 的转移有关，HCC 组织中 OPN 的阳性率（70.00%）明显高于癌旁组织和正常肝组织（P<0.01）。在 HCC 细胞系中，OPN 耗竭可减少体外形成的集落和转移数量。总之，HCC 组织中 OPN 的表达与转移或转移有关。特异性 siRNA 可降低 OPN 在 mRNA 和蛋白质水平的表达，并减弱肝癌细胞的侵袭性，提示 OPN 可能是治疗 HCC 转移和复发的有前途的药物。
