Korkmazer Engin, Ustunyurt Emin, Tekin Başar, Cilingir Oguz
Department of Obstetrics and Gynecology, Faculty of Medicine, Eskişehir Osmangazi University, Eskisehir;
Exp Ther Med. 2013 May;5(5):1408-1410. doi: 10.3892/etm.2013.997. Epub 2013 Mar 11.
The role of apolipoprotein E (Apo E) gene polymorphisms in the etiology of recurrent pregnancy loss (RPL) is not clearly understood. We evaluated this polymorphism in unexplained pregnancy losses in a group of Turkish women. In our prospective case-control study, 45 well-characterized RPL cases were examined for their Apo E genotypes, based on restriction fragment length polymorphism analysis of polymerase chain reaction (PCR)-amplified fragments. The observed genotypes were compared with those obtained from equal number matched controls. We observed similar Apo E genotypes and E2, E3 and E4 allele frequency distribution among RPL patients and controls. The allele frequencies obtained in patients and controls, respectively, were as follows: E2=8 (9%) and 12 (13.4%) (P=0.342), E3=66 (73.3%) and 60 (66.6%) (P=0.328) and E4=16 (17.7%) and 18 (20%) (P=0.703). Our data did not support the association of Apo E gene polymorphisms with RPL as reported by previous studies. We endorse adequate characterization of RPL cases and adequate sample size prior to addressing such studies.
载脂蛋白E（Apo E）基因多态性在复发性流产（RPL）病因学中的作用尚不清楚。我们评估了一组土耳其女性不明原因流产中的这种多态性。在我们的前瞻性病例对照研究中，基于聚合酶链反应（PCR）扩增片段的限制性片段长度多态性分析，对45例特征明确的RPL病例进行了Apo E基因型检测。将观察到的基因型与从数量相等的匹配对照中获得的基因型进行比较。我们在RPL患者和对照中观察到相似的Apo E基因型以及E2、E3和E4等位基因频率分布。患者和对照中分别获得的等位基因频率如下：E2 = 8（9%）和12（13.4%）（P = 0.342），E3 = 66（73.3%）和60（66.6%）（P = 0.328），E4 = 16（17.7%）和18（20%）（P = 0.703）。我们的数据不支持先前研究报道的Apo E基因多态性与RPL的关联。我们支持在开展此类研究之前对RPL病例进行充分的特征描述并确保足够的样本量。
