Goodman Chelsi, Goodman Cyle S, Hur Jee, Jeyendran R S, Coulam Carolyn
CARI Reproductive Institute, Chicago, IL, USA.
Am J Reprod Immunol. 2009 Jan;61(1):34-8. doi: 10.1111/j.1600-0897.2008.00659.x.
We have previously reported the role of polymorphisms of thrombogenic genes involved in coagulation and fibrinolysis as risk factors for recurrent pregnancy loss. Thrombophilia has been viewed as a multigenic disorder rather than a monogenetic clinical phenotype and Apo E has been shown to play an important role in lipid metabolism in pregnancy. As individuals carrying the E4 allele of the ApoE gene have the highest risk for thrombosis, we evaluated the frequency of the Apo E4 genotype among women suffering from recurrent pregnancy loss.
Buccal swabs were obtained from 69 women with a history of two or more consecutive spontaneous abortions and 37 women with at least two live births and not more than one miscarriage. DNA was extracted from the buccal swabs and PCR amplification of Apo E2, E3, and E4 was performed.
Women experiencing recurrent pregnancy loss had a significantly higher prevalence of Apo E3/4, E4/4 genotypes (21.7%) compared with control women (5.4%) (P = 0.036).
Apo E4 polymorphism may contribute to the thrombophilic risk factors contributing to recurrent pregnancy loss.
我们之前曾报道过参与凝血和纤维蛋白溶解的血栓形成基因多态性作为复发性流产风险因素的作用。血栓形成倾向被视为一种多基因疾病而非单基因临床表型，并且已表明载脂蛋白E在妊娠脂质代谢中起重要作用。由于携带载脂蛋白E基因E4等位基因的个体发生血栓形成的风险最高，我们评估了复发性流产女性中载脂蛋白E4基因型的频率。
从69例有两次或更多次连续自然流产史的女性以及37例至少有两次活产且流产不超过一次的女性中获取口腔拭子。从口腔拭子中提取DNA，并对载脂蛋白E2、E3和E4进行PCR扩增。
与对照女性（5.4%）相比，复发性流产女性中载脂蛋白E3/4、E4/4基因型的患病率显著更高（21.7%）（P = 0.036）。
载脂蛋白E4多态性可能是导致复发性流产的血栓形成风险因素之一。
