Sun Xu, Song Maomin, Bai Rixing, Cheng Shi, Xing Ying, Yuan Huisheng, Wang Pilin, Zhou Lisa
Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China ;
Exp Ther Med. 2013 May;5(5):1511-1515. doi: 10.3892/etm.2013.998. Epub 2013 Mar 12.
The aim of this study was to investigate the effects of ileal interposition (IT) on glucose and insulin resistance (IR) in type 2 diabetic mellitus (T2DM), and the role of T-cell factor 7-like 2 (TCF7L2), formerly known as TCF4, in the downregulation of hyperglycemia following IT. Goto-Kakizaki (GK) rats subjected to IT surgery (GK-IT group), GK rats subjected to sham surgery (GK-Sham group) and Wistar (WS) rats subjected to sham surgery (WS-Sham group) were investigated in this study. Fasting plasma glucose, body weight, food intake per 1 kg body weight, insulin and a homeostasis model assessment of insulin resistance (HOMA-IR) were measured pre- and post-surgery. The rats were euthanized 28 days post-surgery and the pancreas of each rat was dissected. The expression levels of TCF7L2 mRNA and protein were analyzed by quantitative RT-PCR and western blotting, respectively. Our results revealed that IT improved both fasting plasma glucose levels and IR in GK rats by upregulating the expression of the TCF7L2 protein. IT provides a valuable therapeutic option for patients with T2DM. Upregulation of TCF7L2 protein expression may be a possible mechanism underlying the improvement of T2DM following IT.
本研究旨在探讨回肠置人术（IT）对2型糖尿病（T2DM）患者血糖及胰岛素抵抗（IR）的影响，以及T细胞因子7样2（TCF7L2，原名TCF4）在IT术后血糖下调中的作用。本研究纳入接受IT手术的Goto-Kakizaki（GK）大鼠（GK-IT组）、接受假手术的GK大鼠（GK-假手术组）和接受假手术的Wistar（WS）大鼠（WS-假手术组）。在手术前后测量空腹血糖、体重、每1kg体重的食物摄入量、胰岛素及胰岛素抵抗稳态模型评估（HOMA-IR）。术后28天对大鼠实施安乐死并解剖每只大鼠的胰腺。分别通过定量逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法分析TCF7L2 mRNA和蛋白质的表达水平。我们的研究结果显示，IT通过上调TCF7L2蛋白的表达改善了GK大鼠的空腹血糖水平和IR。IT为T2DM患者提供了一种有价值的治疗选择。TCF7L2蛋白表达上调可能是IT术后T2DM改善的潜在机制。
