钒在绿色植物中的作用。III. 对小球藻细胞分裂的影响。

The role of vanadium in green plants. III. Influence on cell division of Chlorella.

作者信息

Meisch H U, Benzschawel H

出版信息

Arch Microbiol. 1978 Jan 23;116(1):91-5. doi: 10.1007/BF00408738.

Abstract

Vanadium, although essential for growth and chlorophyll formation in unicellular green algae, reveals toxic influences on cell division of Chlorella pyrenoidosa, these disturbances arising in the same range of V-concentrations as the known positive effects of the trace metal. In permanent light, as documented by cell volume statistics, vanadium (4-10(-7) g-at/1 as NH4VO3) causes a significant shift of the distribution maxima to higher values of the algal cell volume, the shift having its optimum at 10(-5) g-at V/1. It is documented in pH-constant liquid culture that this effect is not due to a change of pH in the nutrient medium. Under synchronous conditions of algal cultivation (16:8h), vanadium causes a total arrest of cell division after 3 periods; this stop lasts for the next 3 cycles. Afterwards, asynchronous divisions newly occur and lead to generally larger autospores. Staining of algal cell nuclei revealed an inhibitory V-effect on nuclear division, yielding giant nuclei with multiple sets of chromosomes, and thereby limiting cell division. Under these conditions, Chlorella pyrenoidosa is not synchronizable in presence of vanadium.

摘要

钒虽然对单细胞绿藻的生长和叶绿素形成必不可少，但对蛋白核小球藻的细胞分裂显示出毒性影响，这些干扰出现在与该痕量金属已知积极作用相同的钒浓度范围内。在持续光照下，通过细胞体积统计记录，钒（以偏钒酸铵计为4×10⁻⁷ g-at/1）会使分布最大值显著向藻类细胞体积的更高值偏移，该偏移在10⁻⁵ g-at V/1时达到最佳。在pH恒定的液体培养中证明，这种效应不是由于营养培养基中pH的变化。在藻类同步培养条件下（16:8小时），钒在3个周期后导致细胞分裂完全停止；这种停止持续到接下来的3个周期。之后，新出现异步分裂并产生通常更大的自体孢子。藻类细胞核染色显示钒对核分裂有抑制作用，产生具有多套染色体的巨型细胞核，从而限制细胞分裂。在这些条件下，如果存在钒，蛋白核小球藻无法实现同步化。