Meisch H U, Bielig H J
Arch Microbiol. 1975 Sep 30;105(1):77-82. doi: 10.1007/BF00447117.
In the presence of vanadium, growth of Scenedesmus obliquus and Chlorella pyrenoidosa was increased five to sixfold as determined by dry weight, when cultured under autotrophic conditions for 7 days. The stimulation by vanadium decreased with increasing stability towards hydrolysis of the iron(III)-compounds added. Pentavalent vanadium (20 mug V/1 as NH4VO3) was able to overcome completely a limited iron-deficiency in the algae following growth in presence of 1.8 - 10(-5) m ferric chloride. Vanadium did not alter the iron uptake into the algal cells. 90% of offered 48V was taken up by Scenedesmus obliquus during 5 days of growth, and 21% thereof were found in the chloroplast fraction. In presence of vanadium, the chlorophyll formation was stimulated in Scenedesmus obliquus. This stimulation by vanadium was found to be light-dependent but occurred to a certain extent in the dark also. The main porphyrin of the yellow mutant 211-11h/20 of Chlorella vulgaris was identified as protoporphyrin-IX. The formation of this compound was stimulated by vanadium within 10 days up to 83%. The role of vanadium in the biosynthesis of chlorophylls is discussed.
在钒存在的情况下，斜生栅藻和蛋白核小球藻在自养条件下培养7天，以干重计其生长增加了五到六倍。随着添加的铁（III）化合物水解稳定性的增加，钒的刺激作用减弱。五价钒（以偏钒酸铵计为20μg V/1）能够完全克服藻类在1.8×10⁻⁵ m氯化铁存在下生长后的有限缺铁情况。钒不会改变藻类细胞对铁的吸收。在生长5天期间，斜生栅藻吸收了所提供的⁴⁸V的90%，其中21%存在于叶绿体部分。在钒存在的情况下，斜生栅藻中的叶绿素形成受到刺激。发现钒的这种刺激作用依赖于光照，但在黑暗中也有一定程度的发生。普通小球藻黄色突变体211 - 11h/20的主要卟啉被鉴定为原卟啉-IX。在10天内，钒对该化合物形成的刺激作用高达83%。本文讨论了钒在叶绿素生物合成中的作用。
