Abbas Muhammad, Munir Muhammad, Khaliq Syed Abdul, Haq Muhammad Ikram Ul, Tanveer Khan Muhammad, Qureshi Zafar Ul Ahsan
Quality Control Laboratory, Veterinary Research Institute, Lahore, Pakistan.
ISRN Vet Sci. 2011 Dec 22;2011:501235. doi: 10.5402/2011/501235. Print 2011.
Paratuberculosis is a chronic bowel disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its secretion through semen highlights the importance of paratuberculosis-free breeding bulls. The breeding and teaser bulls at three semen production units (SPUs) located in Punjab, Pakistan, were screened for the presence of antibodies against MAP. A total of 253 samples were collected from SPUs and a commercially available indirect screen ELISA (Is-ELISA) was applied. Is-ELISA detected antibodies in 20 (24.6%), 16 (22.8%), and 17 (16.6%) samples from SPU-I, SPU-II, and SPU-III, respectively. Collectively, seroprevalence of 20.0% (47/235) in breeding bulls and 33.3% (6/18) in teaser bulls was observed, and thus it poses a potential threat of disease spread to a high number of heifers and cows through artificial insemination. Therefore, this paper highlights the presence of the disease for the first time at SPUs and triggers attempts to ascertain the prevalence of paratuberculosis throughout the country.
副结核病是由鸟分枝杆菌副结核亚种（MAP）引起的反刍动物慢性肠道疾病。通过精液传播该病凸显了无副结核病种公牛的重要性。对位于巴基斯坦旁遮普省的三个精液生产单位（SPU）的种公牛和试情公牛进行了抗MAP抗体筛查。从这些精液生产单位共采集了253份样本，并应用了一种市售的间接筛查酶联免疫吸附测定法（Is-ELISA）。Is-ELISA分别在来自精液生产单位I、精液生产单位II和精液生产单位III的20份（24.6%）、16份（22.8%）和17份（16.6%）样本中检测到了抗体。总体而言，种公牛的血清阳性率为20.0%（47/235），试情公牛的血清阳性率为33.3%（6/18），因此它对通过人工授精将疾病传播给大量小母牛和母牛构成了潜在威胁。因此，本文首次强调了精液生产单位存在该病的情况，并促使人们尝试确定全国副结核病的流行情况。
