Khan Farhan Anwar, Chaudhry Zafar Iqbal, Ali Muhammad Ijaz, Khan Shahid, Mumtaz Naima, Ahmad Ijaz
Centre of Biological Production, Directorate of Veterinary Research Institute, NWFP, Peshawar, 25000, Pakistan.
Trop Anim Health Prod. 2010 Apr;42(4):633-8. doi: 10.1007/s11250-009-9467-8. Epub 2009 Oct 9.
Tissue samples were collected at random from cattle (Bos taurus) and buffalo (Bubalus bubalis) from an abattoir of the district of Lahore and were analyzed for the presence of Mycobacterium avium subsp. paratuberculosis and Mycobacterium bovis through acid-fast staining and polymerase chain reaction (PCR). Body condition of animals and diarrhea were recorded. Most of the animals were emaciated. Diarrhea was noticed in 15.6% of buffaloes and 19.2% of cattle. Intestinal pathology was observed in 29% of buffaloes and 32.8% of cattle. Number of mesenteric lymph node (MLN) showing gross lesions was a bit higher (35.6%) in cattle than buffalo (31.2%). Acid-fast staining of tissue scraping smears revealed the presence of acid-fast bacilli (AFB) in 17.4% intestinal and 16.4% MLN tissue samples in buffalo, while in cattle 19.2% intestinal and 17.8% MLN were found positive for AFB. In buffaloes, PCR confirmed 12.8% intestinal and 12.4% MLN positive samples for M. avium subsp. paratuberculosis. However, in cattle, PCR analysis demonstrated 14.2% positive results for M. avium subsp. paratuberculosis in both MLN and intestinal tissue samples. PCR also confirmed M. bovis in 5.8% of cattle and 5% of buffalo MLN and intestinal tissues. PCR positive tissue samples for M. avium subsp. paratuberculosis were from those animals which were emaciated, having diarrhea, and severe gross lesions. AFB were also detected in tissue scraping smears of these animals. It is concluded that infection by various mycobacterium species can be differentiated by PCR, which is not possible by acid-fast staining technique.
从拉合尔地区一家屠宰场的牛（Bos taurus）和水牛（Bubalus bubalis）中随机采集组织样本，通过抗酸染色和聚合酶链反应（PCR）分析副结核分枝杆菌亚种和牛分枝杆菌的存在情况。记录动物的身体状况和腹泻情况。大多数动物消瘦。在15.6%的水牛和19.2%的牛中发现腹泻。在29%的水牛和32.8%的牛中观察到肠道病理变化。出现肉眼可见病变的肠系膜淋巴结（MLN）数量，牛（35.6%）略高于水牛（31.2%）。组织刮片涂片的抗酸染色显示，水牛17.4%的肠道组织样本和16.4%的MLN组织样本中存在抗酸杆菌（AFB），而牛中19.2%的肠道组织样本和17.8%的MLN组织样本抗酸杆菌检测呈阳性。在水牛中，PCR证实12.8%的肠道样本和12.4%的MLN样本副结核分枝杆菌呈阳性。然而，在牛中，PCR分析显示MLN和肠道组织样本中副结核分枝杆菌的阳性率均为14.2%。PCR还在5.8%的牛和5%的水牛MLN及肠道组织中证实存在牛分枝杆菌。副结核分枝杆菌PCR阳性的组织样本来自消瘦、腹泻且有严重肉眼病变的动物。在这些动物的组织刮片涂片中也检测到了AFB。结论是，通过PCR可以区分各种分枝杆菌物种的感染，而抗酸染色技术无法做到这一点。
