Reproductive Physiology and Embryo Transfer Technology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
In Vitro Cell Dev Biol Anim. 2013 Aug;49(7):486-91. doi: 10.1007/s11626-013-9643-z. Epub 2013 Jun 6.
The present study was conducted to see the in vivo developmental potency of caprine parthenogenetic embryos generated in a modified way. The good quality caprine oocytes were matured in presence of cytochalasin B (CCB) and then activated by 7% ethanol followed by 2 mM 6-dimethyl amino purine (6-DMAP) and embryo development was recorded. Early stage parthenogenetic embryos (two to four cells) were surgically transferred in recipients (10). The pregnancy diagnosis was done by nonreturn to oestrus, ultrasonography (USG), and progesterone estimation. The levels of progesterone were above normal values (1 ng/ml) of pregnancy and fall below the level of pregnancy just before retuned to oestrus. Progesterone profile revealed that out of ten recipients (G1-G10), four goats (G1, G2, G3, and G5) returned to oestrus after 43 ± 7.29 (Mean ± SE) d of embryo transfer and six goats (G4, G6, G7, G8, G9, and G10) did not return to cycle even after 70 d of embryo transfer. In three recipients (G4, G5, and G6), the USG on day 40 revealed that there was fluid filled uterine body with solid fetus-like structure. These might be dead fetus and had started resorption. The progesterone profile also corroborated the assumption of pregnancy in these animals. Authors believe that this may be the first report on in vivo diploid parthenogenetic embryo development in caprine species.
本研究旨在观察通过改良方法生成的山羊孤雌胚胎的体内发育潜能。优质山羊卵母细胞在细胞松弛素 B（CCB）存在下成熟，然后用 7%乙醇激活，接着用 2mM 6-二甲基氨基嘌呤（6-DMAP）激活，并记录胚胎发育情况。早期孤雌胚胎（两到四个细胞）通过手术移植到受体（10）中。通过不再发情、超声检查（USG）和孕酮估计进行妊娠诊断。孕酮水平高于妊娠正常值（1ng/ml），在重新发情前降至妊娠水平以下。孕酮谱显示，在 10 个受体（G1-G10）中，4 只山羊（G1、G2、G3 和 G5）在胚胎移植后 43±7.29（平均值±标准误差）天后重新发情，而 6 只山羊（G4、G6、G7、G8、G9 和 G10）即使在胚胎移植后 70 天也没有重新发情。在 3 个受体（G4、G5 和 G6）中，第 40 天的 USG 显示子宫体充满液体，有胎儿样固体结构。这些可能是死胎，已经开始吸收。孕酮谱也证实了这些动物妊娠的假设。作者认为，这可能是山羊物种体内二倍体孤雌胚胎发育的首次报道。
