Kharche Suresh Dinkar, Goel Anil Kumar, Jindal Satish Kumar, Ranjan Ravi, Rout Pramod Kumar, Agarwal Sudhir Kumar, Goel Puja, Saraswat Sonia, Vijh Ramesh Kumar, Malakar Dhruba, Bag Sadhan, Sarkhel Bikash, Bhanja Subrat Kumar
Central Institute for Research on Goats, Makhdoom, PO Farah, 281122, Mathura, UP, India,
In Vitro Cell Dev Biol Anim. 2014 Dec;50(10):893-8. doi: 10.1007/s11626-014-9740-7. Epub 2014 Oct 1.
The aim of this study is to generate parthenogenetic embryos from chemically activated in vitro matured caprine oocytes and to study the in vivo developmental potency of such embryos. The parthenogenetic embryos (2-8 and 16 cells to morula stage) were surgically transferred in 26 recipients. Pregnancy in recipients following embryo transfer was monitored by ultrasonography. The recipient aborted a foetus on day 34 post transfer. Sexing of parthenogenetic foetus showed a single band of amelogenin gene indicating female cell DNA. Microsatellite analysis revealed that the recipient has not contributed genetically to the parthenogenetic foetus confirming the identity of aborted foetus of parthenogenetic origin. The authors believe that this is the first authentic report on in vivo development of parthenogenetic foetus in Capra hircus.
本研究的目的是从化学激活的体外成熟山羊卵母细胞中产生孤雌生殖胚胎,并研究此类胚胎的体内发育潜能。将孤雌生殖胚胎(2-8细胞、16细胞至桑椹胚阶段)通过手术移植到26只受体动物体内。胚胎移植后通过超声检查监测受体动物的妊娠情况。受体动物在移植后第34天流产了一个胎儿。对孤雌生殖胎儿进行性别鉴定,显示牙釉蛋白基因有一条带,表明为雌性细胞DNA。微卫星分析表明,受体动物在基因上未对孤雌生殖胎儿有所贡献,从而证实了流产胎儿为孤雌生殖起源。作者认为,这是关于山羊孤雌生殖胎儿体内发育的第一份可靠报告。