Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA;
Genome Res. 2013 Oct;23(10):1663-74. doi: 10.1101/gr.152538.112. Epub 2013 Jun 5.
Cytosine DNA methylation is one avenue for passing information through cell divisions. Here, we present epigenomic analyses of soybean recombinant inbred lines (RILs) and their parents. Identification of differentially methylated regions (DMRs) revealed that DMRs mostly cosegregated with the genotype from which they were derived, but examples of the uncoupling of genotype and epigenotype were identified. Linkage mapping of methylation states assessed from whole-genome bisulfite sequencing of 83 RILs uncovered widespread evidence for local methylQTL. This epigenomics approach provides a comprehensive study of the patterns and heritability of methylation variants in a complex genetic population over multiple generations, paving the way for understanding how methylation variants contribute to phenotypic variation.
胞嘧啶 DNA 甲基化是通过细胞分裂传递信息的一种途径。在这里，我们对大豆重组自交系 (RIL)及其亲本进行了表观基因组分析。差异甲基化区域 (DMR)的鉴定表明，DMR 主要与它们来源的基因型共分离，但也鉴定出了基因型和表型分离的例子。对 83 个 RIL 进行全基因组亚硫酸氢盐测序评估的甲基化状态的连锁作图揭示了广泛存在的局部甲基 QTL 的证据。这种表观基因组学方法为在复杂遗传群体中通过多个世代对甲基化变体的模式和遗传进行全面研究铺平了道路，为了解甲基化变体如何导致表型变异奠定了基础。
