Cao Yong, Wang Lie, Chen Hong, Lv Zhiqian
No 6th People's Hospital and Department of Pharmacology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
Rev Bras Cir Cardiovasc. 2013 Mar;28(1):54-60. doi: 10.5935/1678-9741.20130009.
A simple method to reduce the ischemia/reperfusion injury that can accompany cardiac surgery would have great clinical value. This study was to investigate the effect of hyperosmotic perfusion on ischemia/reperfusion injury in isolated perfused rat hearts.
Forty male Sprague-Dawley rats were randomly divided either to have their isolated hearts perfused with normal osmotic buffer or buffer made hyperosmotic by addition of glucose. Hearts were then subjected to 30 min ischemia followed by 30 min reperfusion. Coronary flow, time to ischemic arrest, reperfusion arrhythmia, and ventricular function were recorded. Creatine phosphokinase leakage into the coronary artery, and myocardial content and activity of superoxide dismutase and catalase were also examined.
Rat hearts with hyperosmotic perfusion showed higher coronary flow, a prolonged time to ischemic arrest (10.60 vs. 5.63 min, P<0.005), a lower reperfusion arrthythmia score (3.2 vs. 5.3, P<0.001), better ventricular function, and less creatine phosphokinase leakage (340.1 vs. 861.9, P<0.001) than normal osmotic controls. Myocardial catalase content and activity were increased significantly (1435 vs. 917 U/g wet weight, P<0.001) in hearts perfused with hyperosmotic solution in comparison to the normal osmotic controls.
Pretreatment with hyperosmotic perfusion in normal rat hearts, which is attributed partly to the increased antioxidative activity, could provide beneficial effects from ischemia and reperfusion-induced injury by increasing coronary flow, and decreasing reperfusion arrhythmia.
一种能减轻心脏手术中可能伴随的缺血/再灌注损伤的简单方法具有重大临床价值。本研究旨在探讨高渗灌注对离体灌注大鼠心脏缺血/再灌注损伤的影响。
40只雄性Sprague-Dawley大鼠被随机分为两组，一组用等渗缓冲液灌注离体心脏，另一组用添加葡萄糖制成的高渗缓冲液灌注。然后使心脏经历30分钟缺血，接着再灌注30分钟。记录冠状动脉血流量、缺血性停搏时间、再灌注心律失常和心室功能。还检测了肌酸磷酸激酶向冠状动脉的渗漏以及心肌中超氧化物歧化酶和过氧化氢酶的含量与活性。
与等渗对照组相比，高渗灌注的大鼠心脏显示出更高的冠状动脉血流量、更长的缺血性停搏时间（10.60对5.63分钟，P<0.005）、更低的再灌注心律失常评分（3.2对5.3，P<0.001）、更好的心室功能以及更少的肌酸磷酸激酶渗漏（340.1对861.9，P<0.001）。与等渗对照组相比，用高渗溶液灌注的心脏中，心肌过氧化氢酶含量和活性显著增加（1435对917 U/g湿重，P<0.001）。
正常大鼠心脏进行高渗灌注预处理，部分归因于抗氧化活性增加，可通过增加冠状动脉血流量和减少再灌注心律失常，对缺血和再灌注诱导的损伤产生有益作用。
