Lucas Márcio Luís, Carraro Cristina Campos, Belló-Klein Adriane, Kalil Antonio Nocchi, Aerts Newton
Santa Casa de Porto Alegre, Porto Alegre, RS, Brazil.
Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Braz J Cardiovasc Surg. 2016 Nov-Dec;31(6):428-433. doi: 10.5935/1678-9741.20160086.
Oxidative stress seems to be a role in the atherosclerosis process, but research in human beings is scarce.
To evaluate the role of oxidative stress on human aortas of patients submitted to surgical treatment for advanced aortoiliac occlusive disease.
Twenty-six patients were divided into three groups: control group (n=10) formed by cadaveric organ donors; severe aortoiliac stenosis group (patients with severe aortoiliac stenosis; n=9); and total aortoiliac occlusion group (patients with chronic total aortoiliac occlusion; n=7). We evaluated the reactive oxygen species concentration, nicotinamide adenine dinucleotide phosphate-oxidase, superoxide dismutase and catalase activities as well as nitrite levels in samples of aortas harvested during aortofemoral bypass for treatment of advanced aortoiliac occlusive disease.
We observed a higher level of reactive oxygen species in total aortoiliac occlusion group (48.3±9.56 pmol/mg protein) when compared to severe aortoiliac stenosis (33.5±7.4 pmol/mg protein) and control (4.91±0.8 pmol/mg protein) groups (P<0.05). Nicotinamide adenine dinucleotide phosphate oxidase activity was also higher in total aortoiliac occlusion group when compared to the control group (3.81±1.7 versus 1.05±0.31 µmol/min.mg protein; P<0.05). Furthermore, superoxide dismutase and catalase activities were significantly higher in the severe aortoiliac stenosis and total aortoiliac occlusion groups when compared to the control cases (P<0.05). Nitrite concentration was smaller in the severe aortoiliac stenosis group in comparing to the other groups.
Our results indicated an increase of reactive oxygen species levels and nicotinamide adenine dinucleotide phosphate-oxidase activity in human aortic samples of patients with advanced aortoiliac occlusive disease. The increase of antioxidant enzymes activities may be due to a compensative phenomenon to reactive oxygen species production mediated by nicotinamide adenine dinucleotide phosphate oxidase. This preliminary study offers us a more comprehensive knowledge about the role of oxidative stress in advanced aortoiliac occlusive disease in human beings.
氧化应激似乎在动脉粥样硬化过程中起作用，但人类研究较少。
评估氧化应激在接受晚期主-髂动脉闭塞性疾病手术治疗患者的人体主动脉中的作用。
26例患者分为三组：由尸体器官供体组成的对照组（n = 10）；严重主-髂动脉狭窄组（严重主-髂动脉狭窄患者；n = 9）；以及主-髂动脉完全闭塞组（慢性主-髂动脉完全闭塞患者；n = 7）。我们评估了在治疗晚期主-髂动脉闭塞性疾病的股动脉旁路手术期间采集的主动脉样本中的活性氧浓度、烟酰胺腺嘌呤二核苷酸磷酸氧化酶、超氧化物歧化酶和过氧化氢酶活性以及亚硝酸盐水平。
与严重主-髂动脉狭窄组（33.5±7.4 pmol/mg蛋白质）和对照组（4.91±0.8 pmol/mg蛋白质）相比，我们观察到主-髂动脉完全闭塞组的活性氧水平更高（48.3±9.56 pmol/mg蛋白质）（P<0.05）。与对照组相比，主-髂动脉完全闭塞组的烟酰胺腺嘌呤二核苷酸磷酸氧化酶活性也更高（3.81±1.7对1.05±0.31 μmol/min.mg蛋白质；P<0.05）。此外，与对照病例相比，严重主-髂动脉狭窄组和主-髂动脉完全闭塞组的超氧化物歧化酶和过氧化氢酶活性显著更高（P<0.05）。与其他组相比，严重主-髂动脉狭窄组的亚硝酸盐浓度较低。
我们的结果表明，晚期主-髂动脉闭塞性疾病患者的人体主动脉样本中活性氧水平和烟酰胺腺嘌呤二核苷酸磷酸氧化酶活性增加。抗氧化酶活性的增加可能是由于烟酰胺腺嘌呤二核苷酸磷酸氧化酶介导的活性氧产生的补偿现象。这项初步研究为我们提供了关于氧化应激在人类晚期主-髂动脉闭塞性疾病中的作用的更全面的知识。
