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苹果花叶病毒宿主偏好的外壳蛋白变异性?

Reflects the coat protein variability of apple mosaic virus host preference?

作者信息

Grimová L, Winkowska L, Ryšánek P, Svoboda P, Petrzik K

机构信息

Department of Crop Protection, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 21, Prague 6, Czech Republic.

出版信息

Virus Genes. 2013 Aug;47(1):119-25. doi: 10.1007/s11262-013-0925-z. Epub 2013 Jun 6.

Abstract

Apple mosaic virus (ApMV) is a widespread ssRNA virus which infects diverse species of Rosales. The phylogenetic analysis of complete capsid protein gene of the largest set of ApMV isolates discriminated two main clusters of isolates: one cluster correlates with Maloideae hosts and Trebouxia lichen algae hosts; a second with hop, Prunus, and other woody tree hosts. No correlation was found between clusters and geographic origin of virus isolates, and positive selection hypothesis in distinct hosts was not confirmed: in all virus populations, purifying selection had occurred. GGT→AAT substitution resulted in Gly→Asn change inside the zinc-finger motif in the capsid protein was revealed specific for discrimination of the clusters and we hypothesise that could influence the host preference.

摘要

苹果花叶病毒(ApMV)是一种广泛传播的单链RNA病毒,可感染蔷薇目多种植物。对最大一组ApMV分离株的完整衣壳蛋白基因进行系统发育分析,区分出两个主要的分离株簇:一个簇与苹果亚科宿主和橘色藻属地衣藻类宿主相关;另一个与啤酒花、李属及其他木本树宿主相关。未发现簇与病毒分离株地理起源之间存在相关性,且未证实不同宿主中的正选择假说:在所有病毒群体中均发生了纯化选择。发现GGT→AAT替换导致衣壳蛋白锌指基序内的甘氨酸→天冬酰胺变化,这对于区分簇具有特异性,我们推测这可能影响宿主偏好。

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