College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, People's Republic of China.
Biol Trace Elem Res. 2013 Jul;154(1):156-66. doi: 10.1007/s12011-013-9678-8. Epub 2013 Jun 6.
Oxidative stress is well documented to cause injury to endothelial cells (ECs), which in turn trigger cardiovascular diseases. Previous studies revealed that cerium oxide nanoparticles (nanoceria) had antioxidant property, but the protective effect of nanoceria on ROS injury to ECs and cardiovascular diseases has not been reported. In the current study, we investigated the protective effect and underlying mechanisms of nanoceria on oxidative injury to ECs. The cell viability, lactate dehydrogenase release, cellular uptake, intracellular localization and reactive oxygen species (ROS) levels, endocytosis mechanism, cell apoptosis, and mitochondrial membrane potential were performed. The results indicated that nanoceria had no cytotoxicity on ECs but had the ability to prevent injury by H2O2. Nanoceria could be uptaken into ECs through caveolae- and clathrin-mediated endocytosis and distributed throughout the cytoplasma. The internalized nanoceria effectively attenuated ROS overproduction induced by H2O2. Apoptosis was also alleviated greatly by nanoceria pretreatment. These results may be helpful for more rational application of nanoceria in biomedical fields in the future.
氧化应激被充分证明会导致内皮细胞（ECs）损伤，进而引发心血管疾病。先前的研究表明，氧化铈纳米粒子（纳米 CeO2）具有抗氧化特性，但纳米 CeO2 对 ECs 中 ROS 损伤和心血管疾病的保护作用尚未见报道。在本研究中，我们研究了纳米 CeO2 对 ECs 氧化损伤的保护作用及其潜在机制。进行了细胞活力、乳酸脱氢酶释放、细胞摄取、细胞内定位和活性氧（ROS）水平、内吞作用机制、细胞凋亡和线粒体膜电位测定。结果表明，纳米 CeO2 对 ECs 无细胞毒性，但具有预防 H2O2 损伤的能力。纳米 CeO2 可通过 caveolae 和网格蛋白介导的内吞作用被摄取到 ECs 中，并分布于整个细胞质。内化的纳米 CeO2 可有效抑制 H2O2 诱导的 ROS 过度产生。纳米 CeO2 预处理也大大减轻了细胞凋亡。这些结果可能有助于将来更合理地将纳米 CeO2 应用于生物医学领域。
