混合验证：一种简便、可还原的聚乙二醇化方法，可快速筛选体内潜在的治疗性蛋白质。
Mix to validate: a facile, reversible PEGylation for fast screening of potential therapeutic proteins in vivo.
机构信息
Russell H. Morgan Department of Radiology and Radiological Science, Center for Cancer Nanotechnology Excellence, Johns Hopkins University, 400 North Broadway, Baltimore, MD 21231, USA.
出版信息
Angew Chem Int Ed Engl. 2013 Jul 1;52(27):6880-4. doi: 10.1002/anie.201302181. Epub 2013 Jun 5.
Abstract
: To advance the rate of novel protein therapies entering the clinic, we provide researchers a facile tool for protein drug efficacy testing in animal models in a high throughput manner. Here, we utilize the concept of PEGylating proteins through complementary interactions between His-tag and Ni complex of NTA, a well-established practice in protein research, to improve blood half-life of therapeutic protein candidates after systemic administration .
摘要
为了提高新型蛋白质疗法进入临床的速度，我们为研究人员提供了一种在动物模型中高通量进行蛋白质药物功效测试的简便工具。在这里，我们利用 His 标签和 Ni 配合物之间的互补相互作用将蛋白质 PEGylation，这是蛋白质研究中一种成熟的做法，以提高治疗性蛋白质候选物在系统给药后的血液半衰期。
相似文献
1
Mix to validate: a facile, reversible PEGylation for fast screening of potential therapeutic proteins in vivo.混合验证：一种简便、可还原的聚乙二醇化方法，可快速筛选体内潜在的治疗性蛋白质。
Angew Chem Int Ed Engl. 2013 Jul 1;52(27):6880-4. doi: 10.1002/anie.201302181. Epub 2013 Jun 5.
2
PEGylation and its impact on the design of new protein-based medicines.聚乙二醇化及其对新型蛋白质类药物设计的影响。
Future Med Chem. 2014;6(16):1829-46. doi: 10.4155/fmc.14.125.
3
Noncovalent PEGylation, An Innovative Subchapter in the Field of Protein Modification.非共价聚乙二醇化，蛋白质修饰领域的一个创新分支。
J Pharm Sci. 2016 Feb;105(2):386-390. doi: 10.1002/jps.24692. Epub 2016 Jan 11.
4
Obstacles and pitfalls in the PEGylation of therapeutic proteins.治疗性蛋白质聚乙二醇化中的障碍与陷阱。
Curr Opin Drug Discov Devel. 2008 Mar;11(2):242-50.
5
PEGylation of therapeutic proteins.治疗性蛋白的聚乙二醇化。
Biotechnol J. 2010 Jan;5(1):113-28. doi: 10.1002/biot.200900218.
6
The immunogenicity of polyethylene glycol: facts and fiction.聚乙二醇的免疫原性：事实与虚构。
Pharm Res. 2013 Jul;30(7):1729-34. doi: 10.1007/s11095-013-1067-7. Epub 2013 May 15.
7
Discussion about several potential drawbacks of PEGylated therapeutic proteins.讨论聚乙二醇化治疗性蛋白的几个潜在缺点。
Biol Pharm Bull. 2014;37(3):335-9. doi: 10.1248/bpb.b13-00661. Epub 2013 Dec 12.
8
The impact of PEGylation on biological therapies.聚乙二醇化对生物疗法的影响。
BioDrugs. 2008;22(5):315-29. doi: 10.2165/00063030-200822050-00004.
9
Noncovalent PEGylation of L-asparaginase using PEGylated polyelectrolyte.使用聚乙二醇化聚电解质对L-天冬酰胺酶进行非共价聚乙二醇化修饰。
J Pharm Sci. 2015 Feb;104(2):587-92. doi: 10.1002/jps.24217. Epub 2014 Oct 29.
10
Site-specific modification and PEGylation of pharmaceutical proteins mediated by transglutaminase.转谷氨酰胺酶介导的药用蛋白质的位点特异性修饰和聚乙二醇化
Adv Drug Deliv Rev. 2008 Jan 3;60(1):13-28. doi: 10.1016/j.addr.2007.06.015. Epub 2007 Aug 16.
引用本文的文献
1
Stable colonization of Akkermansia muciniphila educates host intestinal microecology and immunity to battle against inflammatory intestinal diseases.阿克曼氏菌（Akkermansia muciniphila）的稳定定植可调节宿主肠道微生态和免疫，以抵御炎症性肠道疾病。
Exp Mol Med. 2023 Jan;55(1):55-68. doi: 10.1038/s12276-022-00911-z. Epub 2023 Jan 4.
2
Molecular conjugation using non-covalent click chemistry.使用非共价点击化学的分子共轭
Nat Rev Chem. 2019 Jun;3(6):393-400. doi: 10.1038/s41570-019-0095-1. Epub 2019 Apr 24.
3
Site-Specific PEGylation of Therapeutic Proteins.治疗性蛋白质的位点特异性聚乙二醇化
Int J Mol Sci. 2015 Oct 28;16(10):25831-64. doi: 10.3390/ijms161025831.
本文引用的文献
1
Site-specific PEGylation at histidine tags.组氨酸标签位点特异性聚乙二醇化。
Bioconjug Chem. 2012 Feb 15;23(2):248-63. doi: 10.1021/bc200530x. Epub 2012 Feb 6.
2
State of the art in PEGylation: the great versatility achieved after forty years of research.聚乙二醇化的最新进展：四十年研究取得的巨大多功能性。
J Control Release. 2012 Jul 20;161(2):461-72. doi: 10.1016/j.jconrel.2011.10.037. Epub 2011 Nov 7.
3
PEGylated TNF-related apoptosis-inducing ligand (TRAIL) for effective tumor combination therapy.聚乙二醇化 TNF 相关凋亡诱导配体（TRAIL）用于有效的肿瘤联合治疗。
Biomaterials. 2011 Nov;32(33):8529-37. doi: 10.1016/j.biomaterials.2011.07.051. Epub 2011 Aug 19.
4
PEGylated TNF-related apoptosis-inducing ligand (TRAIL) analogues: pharmacokinetics and antitumor effects.聚乙二醇化 TNF 相关凋亡诱导配体（TRAIL）类似物：药代动力学和抗肿瘤作用。
Bioconjug Chem. 2011 Aug 17;22(8):1631-7. doi: 10.1021/bc200187k. Epub 2011 Jul 22.
5
Multifunctional Poly(ethylene glycol)s.多功能聚乙二醇。
Angew Chem Int Ed Engl. 2011 Aug 22;50(35):7988-97. doi: 10.1002/anie.201100027. Epub 2011 Jul 12.
6
TRAIL-mediated signaling in prostate, bladder and renal cancer.TRAIL 介导的前列腺癌、膀胱癌和肾癌信号通路。
Nat Rev Urol. 2011 Jun 14;8(8):417-27. doi: 10.1038/nrurol.2011.81.
7
Multivalent and flexible PEG-nitrilotriacetic acid derivatives for non-covalent protein pegylation.多价且灵活的聚乙二醇-氮川三乙酸衍生物用于非共价蛋白质 PEG 化。
Pharm Res. 2011 Oct;28(10):2412-21. doi: 10.1007/s11095-011-0468-8. Epub 2011 May 25.
8
Role of hexahistidine in directed nanoassemblies of tobacco mosaic virus coat protein.六组氨酸在烟草花叶病毒外壳蛋白定向纳米组装中的作用。
ACS Nano. 2011 Mar 22;5(3):1606-16. doi: 10.1021/nn1025719. Epub 2011 Feb 25.
9
Poly(ethylene glycol) in drug delivery: pros and cons as well as potential alternatives.聚乙二醇在药物传递中的应用：优缺点及潜在替代品。
Angew Chem Int Ed Engl. 2010 Aug 23;49(36):6288-308. doi: 10.1002/anie.200902672.
10
Improved antitumor activity and tumor targeting of NH(2)-terminal-specific PEGylated tumor necrosis factor-related apoptosis-inducing ligand.靶向肿瘤坏死因子相关凋亡诱导配体氨基端的聚乙二醇化修饰增强其抗肿瘤活性和肿瘤靶向性。
Mol Cancer Ther. 2010 Jun;9(6):1719-29. doi: 10.1158/1535-7163.MCT-09-1076. Epub 2010 Jun 1.
Zotero 插件