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比较不同检测方法以评估人乳头瘤病毒(HPV)感染和接种疫苗后16型和18型HPV特异性抗体。

Comparison of different assays to assess human papillomavirus (HPV) type 16- and 18-specific antibodies after HPV infection and vaccination.

作者信息

Scherpenisse Mirte, Schepp Rutger M, Mollers Madelief, Mooij Sofie H, Meijer Chris J L M, Berbers Guy A M, van der Klis Fiona R M

机构信息

Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Bilthoven, the Netherlands.

出版信息

Clin Vaccine Immunol. 2013 Aug;20(8):1329-32. doi: 10.1128/CVI.00153-13. Epub 2013 Jun 5.

Abstract

We compared the measurement of human papillomavirus (HPV)-specific serum antibody levels with the virus-like-particle multiplex immunoassay (VLP-MIA), competitive Luminex immunoassay (cLIA), and glutathione S-transferase (GST) L1-based MIA. Using a large panel of serum samples, these assays showed mutually good correlations for both naturally induced and vaccine-derived HPV-specific antibody levels. However, an adaptation of the GST L1-based MIA resulted in an improved correlation with both cLIA and VLP-MIA.

摘要

我们将人乳头瘤病毒(HPV)特异性血清抗体水平的检测方法与病毒样颗粒多重免疫测定法(VLP-MIA)、竞争性Luminex免疫测定法(cLIA)以及基于谷胱甘肽S-转移酶(GST)L1的免疫测定法进行了比较。使用大量血清样本,这些检测方法在自然诱导和疫苗衍生的HPV特异性抗体水平方面均显示出良好的相互相关性。然而,对基于GST L1的免疫测定法进行改进后,其与cLIA和VLP-MIA的相关性均得到了提高。

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