Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota 57007, USA.
Prep Biochem Biotechnol. 2013;43(6):601-11. doi: 10.1080/10826068.2012.762929.
Citric acid was produced by five species of the yeast Candida after growth on a medium containing soy biodiesel-based crude glycerol. After growth on a medium containing 10 g L(-1) or 60 g L(-1) crude glycerol for 168 hr at 30°C, Candida parapsilosis ATCC 7330 and C. guilliermondii ATCC 9058 produced the highest citric acid levels. On 10 g L(-1) or 60 g L(-1) crude glycerol for 168 hr at 30°C, the citric acid level produced by C. parapsilosis ATCC 7330 was 1.8 g L(-1) or 11.3 g L(-1), respectively, while C. guilliermondii ATCC 9058 produced citric acid concentrations of 3.0 g L(-1) or 10.4 g L(-1), respectively. Biomass production by C. guilliermondii ATCC 9058 on 10 g L(-1) or 60 g L(-1) crude glycerol for 168 hr at 30°C was highest at 1.2 g L(-1) or 6.9 g L(-1), respectively. The citric acid yields observed for C. guilliermondii ATCC 9058 after growth on 10 g L(-1) or 60 g L(-1) crude glycerol (0.35 g g(-1) or 0.21 g g(-1), respectively) were generally higher than for the other Candida species tested. When similar crude glycerol concentrations were present in the culture medium, citric acid yields observed for some of the Candida species utilized in this study were about the same or higher compared to citric acid yields by Yarrowia lipolytica strains. Based on the findings, it appeared that C. guilliermondii ATCC 9058 was the most effective species utilized, with its citric acid production being similar to what has been observed when citric acid-producing strains of Y. lipolytica were grown on crude glycerol under batch conditions that could be of significance to biobased citric acid production.
五种酵母属 Candida 种在含有大豆生物柴油粗甘油的培养基上生长后生产柠檬酸。在 30°C 下用 10 g/L(-1)或 60 g/L(-1)粗甘油培养 168 小时后，Candida parapsilosis ATCC 7330 和 C. guilliermondii ATCC 9058 产生的柠檬酸水平最高。在 10 g/L(-1)或 60 g/L(-1)粗甘油中于 30°C 培养 168 小时后，C. parapsilosis ATCC 7330 产生的柠檬酸水平分别为 1.8 g/L(-1)或 11.3 g/L(-1)，而 C. guilliermondii ATCC 9058 产生的柠檬酸浓度分别为 3.0 g/L(-1)或 10.4 g/L(-1)。C. guilliermondii ATCC 9058 在 10 g/L(-1)或 60 g/L(-1)粗甘油中于 30°C 培养 168 小时的生物量最高，分别为 1.2 g/L(-1)或 6.9 g/L(-1)。在生长于 10 g/L(-1)或 60 g/L(-1)粗甘油后，C. guilliermondii ATCC 9058 的柠檬酸产率分别为 0.35 g g(-1)或 0.21 g g(-1)，通常高于其他测试的 Candida 种。当培养基中存在类似的粗甘油浓度时，与 Yarrowia lipolytica 菌株在分批条件下生长时的柠檬酸产率相比，本研究中使用的一些 Candida 种的柠檬酸产率相同或更高。根据研究结果，似乎 C. guilliermondii ATCC 9058 是利用效率最高的物种，其柠檬酸生产与柠檬酸产生菌株在批量条件下生长时的柠檬酸生产相似，这可能对生物基柠檬酸生产具有重要意义。
